論文 - 詳細
| RRC ID | 11831 |
|---|---|
| 著者 | Inoue K, Kohda T, Sugimoto M, Sado T, Ogonuki N, Matoba S, Shiura H, Ikeda R, Mochida K, Fujii T, Sawai K, Otte AP, Tian XC, Yang X, Ishino F, Abe K, Ogura A. |
| タイトル | Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer. |
| ジャーナル | Science |
| Abstract |
Cloning mammals by means of somatic cell nuclear transfer (SCNT) is highly inefficient because of erroneous reprogramming of the donor genome. Reprogramming errors appear to arise randomly, but the nature of nonrandom, SCNT-specific errors remains elusive. We found that Xist, a noncoding RNA that inactivates one of the two X chromosomes in females, was ectopically expressed from the active X (Xa) chromosome in cloned mouse embryos of both sexes. Deletion of Xist on Xa showed normal global gene expression and resulted in about an eight- to ninefold increase in cloning efficiency. We also identified an Xist-independent mechanism that specifically down-regulated a subset of X-linked genes through somatic-type repressive histone blocks. Thus, we have identified nonrandom reprogramming errors in mouse cloning that can be altered to improve the efficiency of SCNT methods. |
| 巻・号 | 330(6003) |
| ページ | 496-9 |
| 公開日 | 2010-10-22 |
| DOI | 10.1126/science.1194174 |
| PII | science.1194174 |
| PMID | 20847234 |
| MeSH | Animals Cloning, Organism / methods* Down-Regulation Embryo, Mammalian / metabolism Female Gene Deletion Gene Expression Profiling Male Mice Nuclear Transfer Techniques* RNA, Long Noncoding RNA, Untranslated / biosynthesis RNA, Untranslated / genetics RNA, Untranslated / physiology* X Chromosome* |
| IF | 41.846 |
| 引用数 | 152 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 実験動物マウス | RBRC01260 |