RRC ID 11839
Author Song JW, Misgeld T, Kang H, Knecht S, Lu J, Cao Y, Cotman SL, Bishop DL, Lichtman JW.
Title Lysosomal activity associated with developmental axon pruning.
Journal J. Neurosci.
Abstract Clearance of cellular debris is a critical feature of the developing nervous system, as evidenced by the severe neurological consequences of lysosomal storage diseases in children. An important developmental process, which generates considerable cellular debris, is synapse elimination, in which many axonal branches are pruned. The fate of these pruned branches is not known. Here, we investigate the role of lysosomal activity in neurons and glia in the removal of axon branches during early postnatal life. Using a probe for lysosomal activity, we observed robust staining associated with retreating motor axons. Lysosomal function was involved in axon removal because retreating axons were cleared more slowly in a mouse model of a lysosomal storage disease. In addition, we found lysosomal activity in the cerebellum at the time of, and at sites where, climbing fibers are eliminated. We propose that lysosomal activity is a central feature of synapse elimination. Moreover, staining for lysosomal activity may serve as a marker for regions of the developing nervous system undergoing axon pruning.
Volume 28(36)
Pages 8993-9001
Published 2008-9-3
DOI 10.1523/JNEUROSCI.0720-08.2008
PII 28/36/8993
PMID 18768693
PMC PMC2693713
MeSH Age Factors Amines / metabolism Animals Animals, Newborn Antineoplastic Agents, Hormonal / pharmacology Axons / metabolism* Axons / ultrastructure* Brain / growth & development* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism In Vitro Techniques Luminescent Proteins / genetics Luminescent Proteins / metabolism Lysosomes / physiology* Membrane Glycoproteins / genetics Membrane Glycoproteins / metabolism Mice Mice, Transgenic Microscopy, Confocal / methods Microscopy, Electron, Transmission / methods Molecular Chaperones / genetics Molecular Chaperones / metabolism Neuromuscular Junction / drug effects Neuromuscular Junction / growth & development Neurons / cytology* Organelles / metabolism S100 Proteins / genetics S100 Proteins / metabolism Tamoxifen / pharmacology
IF 6.074
Times Cited 44
Mice GFP-LC3#53