RRC ID 11953
著者 Fukuda M, Takahashi S, Haramoto Y, Onuma Y, Kim YJ, Yeo CY, Ishiura S, Asashima M.
タイトル Zygotic VegT is required for Xenopus paraxial mesoderm formation and is regulated by Nodal signaling and Eomesodermin.
ジャーナル Int J Dev Biol
Abstract The T-box gene VegT plays a crucial role during mesendoderm specification of the amphibian embryo. While the function of maternal VegT (mVegT) has been extensively investigated, little is known about the function and transcriptional regulation of zygotic VegT (zVegT). In the present study, we used comparative genomics and a knockdown experiment to demonstrate that zVegT is the orthologous gene of zebrafish Spadetail/Tbx16 and chick Tbx6L/Tbx6, and has an essential role in paraxial mesodermal formation. zVegT knockdown embryos show several defects in the patterning of trunk mesoderm, such as abnormal segmentation of somites, a reduction in muscle, and the formation of an abnormal mass of cells at the tail tip. We also identified the cis-regulatory elements of zVegT that are necessary and sufficient for mesoderm-specific expression. These cis-regulatory elements are located in two separate upstream regions of zVegT, corresponding to the first intron of mVegT. The results of in vitro binding and functional assays indicate that Forkhead box H1 (FoxH1) and Eomesodermin (Eomes) are the trans-acting factors required for zVegT expression. Our results highlight the essential role of zVegT in organization of paraxial mesoderm, and reveal that zVegT is regulated by a coherent feedforward loop of Nodal signaling via Eomes.
巻・号 54(1)
ページ 81-92
公開日 2010-1-1
DOI 10.1387/ijdb.082837mf
PII 082837mf
PMID 20013651
MeSH Animals Base Sequence Blotting, Western Body Patterning Chickens Electrophoretic Mobility Shift Assay Embryo, Nonmammalian / metabolism Female Gene Expression Regulation* Immunoenzyme Techniques In Situ Hybridization Mesoderm / physiology* Molecular Sequence Data Nodal Protein / genetics Nodal Protein / metabolism* Promoter Regions, Genetic / genetics Protein Biosynthesis RNA, Messenger / genetics RNA, Messenger / metabolism Regulatory Sequences, Nucleic Acid Reverse Transcriptase Polymerase Chain Reaction Signal Transduction* T-Box Domain Proteins / genetics T-Box Domain Proteins / metabolism* Transcription, Genetic Xenopus Proteins / genetics Xenopus Proteins / metabolism* Xenopus laevis / embryology Zebrafish Zygote / cytology Zygote / metabolism*
IF 1.105
引用数 17
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ツメガエル・イモリ