| RRC ID |
11960
|
| Author |
Hamlet MR, Yergeau DA, Kuliyev E, Takeda M, Taira M, Kawakami K, Mead PE.
|
| Title |
Tol2 transposon-mediated transgenesis in Xenopus tropicalis.
|
| Journal |
Genesis
|
| Abstract |
The diploid frog Xenopus tropicalis is becoming a powerful developmental genetic model system. Sequencing of the X. tropicalis genome is nearing completion and several labs are embarking on mutagenesis screens. We are interested in developing insertional mutagenesis strategies in X. tropicalis. Transposon-mediated insertional mutagenesis, once used exclusively in plants and invertebrate systems, is now more widely applicable to vertebrates. The first step in developing transposons as tools for mutagenesis is to demonstrate that these mobile elements function efficiently in the target organism. Here, we show that the Medaka fish transposon, Tol2, is able to stably integrate into the X. tropicalis genome and will serve as a powerful tool for insertional mutagenesis strategies in the frog.
|
| Volume |
44(9)
|
| Pages |
438-45
|
| Published |
2006-9-1
|
| DOI |
10.1002/dvg.20234
|
| PMID |
16906529
|
| MeSH |
Animals
Base Sequence
Chorionic Gonadotropin / pharmacology
Cysteine / pharmacology
DNA / chemistry
DNA / genetics
DNA Transposable Elements / genetics*
Embryo, Nonmammalian / metabolism
Female
Gene Transfer Techniques*
Green Fluorescent Proteins / metabolism
Humans
Microinjections
Models, Genetic
Molecular Sequence Data
Mutagenesis, Insertional
Oryzias / genetics
Plasmids / administration & dosage
Plasmids / genetics
Promoter Regions, Genetic
Sequence Analysis, DNA
Transgenes
Transposases / genetics
Xenopus / classification
Xenopus / embryology
Xenopus / genetics*
|
| IF |
1.76
|
| Times Cited |
74
|
|
WOS Category
|
GENETICS & HEREDITY
DEVELOPMENTAL BIOLOGY
|
| Resource |
| Clawed frogs / Newts |
|
