Reference - Detail
| RRC ID | 11960 |
|---|---|
| Author | Hamlet MR, Yergeau DA, Kuliyev E, Takeda M, Taira M, Kawakami K, Mead PE. |
| Title | Tol2 transposon-mediated transgenesis in Xenopus tropicalis. |
| Journal | Genesis |
| Abstract |
The diploid frog Xenopus tropicalis is becoming a powerful developmental genetic model system. Sequencing of the X. tropicalis genome is nearing completion and several labs are embarking on mutagenesis screens. We are interested in developing insertional mutagenesis strategies in X. tropicalis. Transposon-mediated insertional mutagenesis, once used exclusively in plants and invertebrate systems, is now more widely applicable to vertebrates. The first step in developing transposons as tools for mutagenesis is to demonstrate that these mobile elements function efficiently in the target organism. Here, we show that the Medaka fish transposon, Tol2, is able to stably integrate into the X. tropicalis genome and will serve as a powerful tool for insertional mutagenesis strategies in the frog. |
| Volume | 44(9) |
| Pages | 438-45 |
| Published | 2006-9-1 |
| DOI | 10.1002/dvg.20234 |
| PMID | 16906529 |
| MeSH | Animals Base Sequence Chorionic Gonadotropin / pharmacology Cysteine / pharmacology DNA / chemistry DNA / genetics DNA Transposable Elements / genetics* Embryo, Nonmammalian / metabolism Female Gene Transfer Techniques* Green Fluorescent Proteins / metabolism Humans Microinjections Models, Genetic Molecular Sequence Data Mutagenesis, Insertional Oryzias / genetics Plasmids / administration & dosage Plasmids / genetics Promoter Regions, Genetic Sequence Analysis, DNA Transgenes Transposases / genetics Xenopus / classification Xenopus / embryology Xenopus / genetics* |
| IF | 1.76 |
| Times Cited | 74 |
| WOS Category | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Clawed frogs / Newts | |