RRC ID 1205
著者 Iwasaki A, Doi T, Umetani M, Watanabe M, Suda M, Hattori Y, Nagoya T.
タイトル Affinity improvement of the high-affinity immunoglobulin E receptor by phage display.
ジャーナル Biochem Biophys Res Commun
Abstract The immunoglobulin E (IgE)-binding site of its high-affinity receptor is localized in the second immunoglobulin-like domain (D2) of the alpha-subunit (Fc epsilon RI alpha). In this study, the randomized pentapeptides were introduced between Glu(132) and Ile(138) of Fc epsilon RI alpha D2 and displayed on a filamentous phage. After eight rounds of panning, a phage clone having a mutation of Asp(135)Tyr(136)Met(137) in Fc epsilon RI alpha D2 was obtained. The binding affinity of the mutant phages to immobilized IgE was approximately 500 times higher than that of the wild type. The mutant phages competitively inhibited the binding of IgE to the soluble receptor at a 50% inhibition (IC(50)) value of 116 pM. The mutant Fc epsilon RI alpha D2, which had been expressed as a fusion protein with glutathione S-transferase in Escherichia coli, also showed higher IgE-binding capacity than the wild type. The mutant Fc epsilon RI alpha D2 is expected to manifest its improved IgE-binding affinity together with any fusion partner.
巻・号 293(1)
ページ 542-8
公開日 2002-4-26
DOI 10.1016/S0006-291X(02)00261-9
PII S0006-291X(02)00261-9
PMID 12054635
MeSH Amino Acid Sequence Amino Acid Substitution Base Sequence Binding Sites Cloning, Molecular Escherichia coli / genetics Humans Immunoglobulin E / metabolism* Kinetics Molecular Sequence Data Mutagenesis Peptide Library* Polymerase Chain Reaction Receptors, IgE / antagonists & inhibitors Receptors, IgE / genetics Receptors, IgE / metabolism* Recombinant Fusion Proteins / metabolism
IF 2.985
引用数 2
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 KU812(RCB0495)