Reference - Detail
| RRC ID | 12093 |
|---|---|
| Author | Hardy ME, Ross LV, Chien CB. |
| Title | Focal gene misexpression in zebrafish embryos induced by local heat shock using a modified soldering iron. |
| Journal | Dev Dyn |
| Abstract |
Misexpression of genes in a temporally and spatially controlled fashion is an important tool for assessing gene function during development. Because few tissue-specific promoters have been identified in zebrafish, inducible systems such as the Cre/LoxP and Tet repressor systems are of limited utility. Here we describe a new method of misexpression: local heat shock using a modified soldering iron. Zebrafish carrying transgenes under the control of a heat shock promoter (hsp70) are focally heated with the soldering iron to induce gene expression in a small area of the embryo. We have validated this method in three stable transgenic lines and at three developmental timepoints. Local heat shock is a fast, easy, and inexpensive method for gene misexpression. |
| Volume | 236(11) |
| Pages | 3071-6 |
| Published | 2007-11-1 |
| DOI | 10.1002/dvdy.21318 |
| PMID | 17907198 |
| MeSH | Animals Animals, Genetically Modified Embryo, Nonmammalian / metabolism* Gene Expression Regulation, Developmental* HSP70 Heat-Shock Proteins / genetics Heat-Shock Response* Hot Temperature* Intercellular Signaling Peptides and Proteins / genetics Nerve Tissue Proteins / genetics Zebrafish / embryology* Zebrafish / genetics |
| IF | 3.275 |
| Times Cited | 24 |
| WOS Category | ANATOMY & MORPHOLOGY DEVELOPMENTAL BIOLOGY |
| Resource | |
| Zebrafish | rw015d (Tg (hsp70:slit2-GFP)) |