| RRC ID |
12138
|
| 著者 |
Yamada-Okabe T, Imamura K, Kawaguchi N, Sakai H, Yamashita M, Matsumoto N.
|
| タイトル |
Functional characterization of the zebrafish WHSC1-related gene, a homolog of human NSD2.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Methylation of specific lysine residues of histone H3 and H4 has been reported to be important in the structuring of chromatin and for the transcription of certain genes. Proteins with SET domains have been shown to methylate specific lysine residues of histone H3 and H4. We isolated a SET domain-containing gene from the zebrafish (Danio rerio). The gene has the highest sequence similarity to human NSD2, also known as Wolf-Hirschhorn syndrome candidate 1 or WHSC1, and therefore, was named DrWhsc1. DrWhsc1 mRNA is expressed in various tissues with the highest level in testis. Morpholino oligonucleotides for the DrWhsc1 gene affected early embryogenesis in zebrafish, such as endbrain enlargement, abnormal cartilage, marked reduction of bone, and incomplete motor neuron formation. Such developmental abnormalities are also observed in Wolf-Hirschhorn syndrome patients and Whsc1-deficient mice. In addition, suppression of the DrWhsc1 gene or defect in the SET domain of DrWhsc1 resulted in impairment of di-methylation of histone H3K36 at early embryogenesis. These results indicate that DrWhsc1 is a functional homolog of WHSC1 and that the SET domain of DrWhsc1 is essential for di-methylation of histone H3K36 in zebrafish.
|
| 巻・号 |
402(2)
|
| ページ |
335-9
|
| 公開日 |
2010-11-12
|
| DOI |
10.1016/j.bbrc.2010.10.027
|
| PII |
S0006-291X(10)01889-9
|
| PMID |
20946879
|
| MeSH |
Animals
Embryo, Nonmammalian / metabolism
Gene Knockdown Techniques
Histone-Lysine N-Methyltransferase / genetics
Histone-Lysine N-Methyltransferase / metabolism*
Histones / metabolism
Humans
Male
Methylation
Protein Structure, Tertiary
Repressor Proteins / genetics
Repressor Proteins / metabolism
Testis / metabolism
Tissue Distribution
Zebrafish / embryology*
Zebrafish / genetics
Zebrafish Proteins / genetics
Zebrafish Proteins / metabolism*
|
| IF |
2.985
|
| 引用数 |
6
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ゼブラフィッシュ |
rw0 (Tg(CM-isl1:GFP)) |
