| RRC ID |
12150
|
| Author |
Tanaka S, Okuno Y, Numazawa S, Yamamoto T, Shioda S, Yoshida T.
|
| Title |
Brain responses to acute withdrawal in phenobarbital-dependent rats.
|
| Journal |
Eur J Pharmacol
|
| Abstract |
Heat shock proteins (HSP) such as HO-1 and HSP27 have been implicated as functioning in a protective manner against oxidative and physical stress. The objective of the current study was to determine the role of HSPs in drug-withdrawal stress induced in phenobarbital-dependent rats. Increased expression of HO-1 and HSP27 was observed in the hippocampus and the cerebral cortex of phenobarbital-withdrawn rats. Gene expression was measured by Northern and Western blot analyses and in situ hybridization. The induction of HO-1 mRNA was suppressed by the administration of the NMDA receptor antagonist, (+)-5-methyl-10,11-dihydro-5H-dibenzo (a,d) cyclohepten-5,10-imine (MK801). Despite significant upregulation of glutamatergic transmission, neuronal cell degeneration was not apparent. These findings suggest that the induction of HO-1 and HSP27 during withdrawal from phenobarbital dependence may play a role in protection against glutamate toxicity.
|
| Volume |
421(2)
|
| Pages |
101-8
|
| Published |
2001-6-8
|
| DOI |
10.1016/s0014-2999(01)01017-2
|
| PII |
S0014-2999(01)01017-2
|
| PMID |
11399265
|
| MeSH |
Animals
Anticonvulsants / adverse effects*
Brain / cytology
Brain / drug effects*
Brain / metabolism
Cell Survival / drug effects
Dizocilpine Maleate / pharmacology
Excitatory Amino Acid Antagonists / pharmacology
Gene Expression Regulation / drug effects
HSP27 Heat-Shock Proteins
Heat-Shock Proteins*
Heme Oxygenase (Decyclizing) / genetics
Heme Oxygenase (Decyclizing) / metabolism
Heme Oxygenase-1
Hippocampus / cytology
Hippocampus / drug effects
Hippocampus / metabolism
In Situ Hybridization
Male
Neoplasm Proteins / genetics
Neoplasm Proteins / metabolism
Phenobarbital / adverse effects*
RNA, Messenger / drug effects
RNA, Messenger / genetics
RNA, Messenger / metabolism
Rats
Rats, Wistar
Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors
Receptors, N-Methyl-D-Aspartate / physiology
Substance Withdrawal Syndrome / metabolism
Substance-Related Disorders
|
| IF |
3.263
|
| Times Cited |
4
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
|
| Resource |
| DNA material |
pRHO1 Rat heme oxygenase cDNA (RDB01208) |
