RRC ID 12188
Author Deguchi J, Makuuchi M, Nakaoka T, Collins T, Takuwa Y.
Title Angiotensin II stimulates platelet-derived growth factor-B chain expression in newborn rat vascular smooth muscle cells and neointimal cells through Ras, extracellular signal-regulated protein kinase, and c-Jun N-terminal protein kinase mechanisms.
Journal Circ Res
Abstract Platelet-derived growth factors (PDGFs) have been implicated in the pathogenesis of vascular proliferative disorders. Vascular smooth muscle cells (VSMCs) are one of the cell types that produce PDGF-B chain in proliferative lesions, although the mechanism of regulation of PDGF-B chain production in these cells is not well understood. In the present study, we demonstrate that angiotensin II (Ang II), which is also implicated in vascular stenosis after angioplasty and atherosclerosis, markedly stimulates PDGF-B chain mRNA expression in cultured newborn rat medial VSMCs and neointimal VSMCs via an AT(1), but not in adult rat VSMCs. In newborn rat VSMCs, Ang II activates extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal protein kinase (JNK), and p38 mitogen-activated protein kinase. The mitogen-activated protein/ERK (MEK) inhibitor PD98059, but not the p38 inhibitor SB203580, abrogates Ang II-induced PDGF-B mRNA expression. Transient transfection analysis using a PDGF-B promoter-luciferase gene reporter construct reveals that Ang II induces transcriptional activation of PDGF-B chain gene, which is abolished by the expression of a dominant negative form of either ERK or JNK, but not of p38. The expression of a dominant negative form of Ras abolishes the stimulatory effects of Ang II on ERK activity and PDGF-B mRNA expression. In adult rat VSMCs, Ang II activates ERK and JNK, but weakly induces Egr-1, a transcription factor implicated in PDGF-B chain gene expression, compared with newborn VSMCs. These data indicate that Ang II activates PDGF-B chain gene expression in VSMCs through mechanisms involving Ras-ERK and JNK.
Volume 85(7)
Pages 565-74
Published 1999-10-1
DOI 10.1161/01.res.85.7.565
PMID 10506481
MeSH Aging / metabolism Angiotensin II / pharmacology* Animals Animals, Newborn / physiology* Calcium-Calmodulin-Dependent Protein Kinases / physiology Cells, Cultured DNA / biosynthesis DNA-Binding Proteins / metabolism Early Growth Response Protein 1 Gene Expression* Genes, ras / physiology Immediate-Early Proteins* JNK Mitogen-Activated Protein Kinases Male Mitogen-Activated Protein Kinases* Muscle, Smooth, Vascular / cytology Muscle, Smooth, Vascular / physiology* Platelet-Derived Growth Factor / genetics* Proto-Oncogene Proteins / genetics* Proto-Oncogene Proteins c-sis RNA, Messenger / metabolism Rats Rats, Wistar Receptor, Angiotensin, Type 1 Receptor, Angiotensin, Type 2 Receptors, Angiotensin / genetics Transcription Factors / metabolism Tunica Intima / cytology Tunica Intima / physiology*
IF 14.467
Times Cited 34
DNA material ETR103 Human Egr-1 cDNA TPA early response gene (RDB01198)