RRC ID 12216
Author Yamashita T, Murakami T, Iida M, Kuwajima M, Shima K.
Title Leptin receptor of Zucker fatty rat performs reduced signal transduction.
Journal Diabetes
Abstract Zucker fatty (fa/fa) rats exhibit overt obesity, hypercholesterolemia, hyperlipidemia, and hyperglycemia as recessive traits. The fa mutation has been determined to be a missense mutation in the extracellular domain of the leptin receptor. We report herein the construction of CHO cells that stably express the fa-type leptin receptor and the characterization of this receptor using mRNA expression levels of the immediate early genes, c-fos, c-jun, and jun-B, which are induced by leptin as a criterion of signal transduction. The fa-type receptor not only exhibits a slightly reduced leptin-binding affinity, but also performs reduced signal transduction.
Volume 46(6)
Pages 1077-80
Published 1997-6
PMID 9166683
MeSH Animals CHO Cells Carrier Proteins / genetics Carrier Proteins / metabolism* Cricetinae Dose-Response Relationship, Drug Gene Expression Regulation / genetics* Genes, Immediate-Early / genetics* Genes, fos / genetics Genes, jun / genetics Iodine Radioisotopes Leptin Proteins / analysis Proteins / metabolism* Proteins / pharmacology* RNA, Messenger / analysis RNA, Messenger / genetics Rats Rats, Zucker Receptors, Cell Surface* Receptors, Leptin Recombinant Proteins / analysis Recombinant Proteins / metabolism Recombinant Proteins / pharmacology Signal Transduction* Transfection / genetics
IF 7.273
Times Cited 95
DNA material 465.20 Mouse JunB (RDB01298)