Reference - Detail
|Author||Yamashita T, Murakami T, Iida M, Kuwajima M, Shima K.|
|Title||Leptin receptor of Zucker fatty rat performs reduced signal transduction.|
Zucker fatty (fa/fa) rats exhibit overt obesity, hypercholesterolemia, hyperlipidemia, and hyperglycemia as recessive traits. The fa mutation has been determined to be a missense mutation in the extracellular domain of the leptin receptor. We report herein the construction of CHO cells that stably express the fa-type leptin receptor and the characterization of this receptor using mRNA expression levels of the immediate early genes, c-fos, c-jun, and jun-B, which are induced by leptin as a criterion of signal transduction. The fa-type receptor not only exhibits a slightly reduced leptin-binding affinity, but also performs reduced signal transduction.
|MeSH||Animals CHO Cells Carrier Proteins / genetics Carrier Proteins / metabolism* Cricetinae Dose-Response Relationship, Drug Gene Expression Regulation / genetics* Genes, Immediate-Early / genetics* Genes, fos / genetics Genes, jun / genetics Iodine Radioisotopes Leptin Proteins / analysis Proteins / metabolism* Proteins / pharmacology* RNA, Messenger / analysis RNA, Messenger / genetics Rats Rats, Zucker Receptors, Cell Surface* Receptors, Leptin Recombinant Proteins / analysis Recombinant Proteins / metabolism Recombinant Proteins / pharmacology Signal Transduction* Transfection / genetics|
|WOS Category||ENDOCRINOLOGY & METABOLISM|
|DNA material||465.20 Mouse JunB (RDB01298)|