Reference - Detail
| RRC ID | 12216 |
|---|---|
| Author | Yamashita T, Murakami T, Iida M, Kuwajima M, Shima K. |
| Title | Leptin receptor of Zucker fatty rat performs reduced signal transduction. |
| Journal | Diabetes |
| Abstract |
Zucker fatty (fa/fa) rats exhibit overt obesity, hypercholesterolemia, hyperlipidemia, and hyperglycemia as recessive traits. The fa mutation has been determined to be a missense mutation in the extracellular domain of the leptin receptor. We report herein the construction of CHO cells that stably express the fa-type leptin receptor and the characterization of this receptor using mRNA expression levels of the immediate early genes, c-fos, c-jun, and jun-B, which are induced by leptin as a criterion of signal transduction. The fa-type receptor not only exhibits a slightly reduced leptin-binding affinity, but also performs reduced signal transduction. |
| Volume | 46(6) |
| Pages | 1077-80 |
| Published | 1997-6-1 |
| DOI | 10.2337/diab.46.6.1077 |
| PMID | 9166683 |
| MeSH | Animals CHO Cells Carrier Proteins / genetics Carrier Proteins / metabolism* Cricetinae Dose-Response Relationship, Drug Gene Expression Regulation / genetics* Genes, Immediate-Early / genetics* Genes, fos / genetics Genes, jun / genetics Iodine Radioisotopes Leptin Proteins / analysis Proteins / metabolism* Proteins / pharmacology* RNA, Messenger / analysis RNA, Messenger / genetics Rats Rats, Zucker Receptors, Cell Surface* Receptors, Leptin Recombinant Proteins / analysis Recombinant Proteins / metabolism Recombinant Proteins / pharmacology Signal Transduction* Transfection / genetics |
| IF | 7.72 |
| Times Cited | 101 |
| WOS Category | ENDOCRINOLOGY & METABOLISM |
| Resource | |
| DNA material | 465.20 Mouse JunB (RDB01298) |