論文 - 詳細
RRC ID | 12216 |
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著者 | Yamashita T, Murakami T, Iida M, Kuwajima M, Shima K. |
タイトル | Leptin receptor of Zucker fatty rat performs reduced signal transduction. |
ジャーナル | Diabetes |
Abstract |
Zucker fatty (fa/fa) rats exhibit overt obesity, hypercholesterolemia, hyperlipidemia, and hyperglycemia as recessive traits. The fa mutation has been determined to be a missense mutation in the extracellular domain of the leptin receptor. We report herein the construction of CHO cells that stably express the fa-type leptin receptor and the characterization of this receptor using mRNA expression levels of the immediate early genes, c-fos, c-jun, and jun-B, which are induced by leptin as a criterion of signal transduction. The fa-type receptor not only exhibits a slightly reduced leptin-binding affinity, but also performs reduced signal transduction. |
巻・号 | 46(6) |
ページ | 1077-80 |
公開日 | 1997-6-1 |
DOI | 10.2337/diab.46.6.1077 |
PMID | 9166683 |
MeSH | Animals CHO Cells Carrier Proteins / genetics Carrier Proteins / metabolism* Cricetinae Dose-Response Relationship, Drug Gene Expression Regulation / genetics* Genes, Immediate-Early / genetics* Genes, fos / genetics Genes, jun / genetics Iodine Radioisotopes Leptin Proteins / analysis Proteins / metabolism* Proteins / pharmacology* RNA, Messenger / analysis RNA, Messenger / genetics Rats Rats, Zucker Receptors, Cell Surface* Receptors, Leptin Recombinant Proteins / analysis Recombinant Proteins / metabolism Recombinant Proteins / pharmacology Signal Transduction* Transfection / genetics |
IF | 7.72 |
引用数 | 101 |
WOS 分野 | ENDOCRINOLOGY & METABOLISM |
リソース情報 | |
遺伝子材料 | 465.20 Mouse JunB (RDB01298) |