| RRC ID |
12229
|
| Author |
Kojima H, Inuzuka S, Miwa T, Furukawa S, Hayashi K, Kanegae Y, Saito I, Ohishi N, Takamori M, Yagi K.
|
| Title |
Construction and characterization of adenoviral vector expressing biologically active brain-derived neurotrophic factor.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
To deliver brain-derived neurotrophic factor (BDNF) to the central nervous system, we sought to attain adenovirus-mediated transfer and expression of the gene in both in vitro and in vivo experiments. For this purpose, we constructed AxCA-BDNF, a recombinant adenoviral vector containing the BDNF cDNA expression cassette. Reverse transcription polymerase chain reaction analyses of the infected HeLa cells and the transduced mouse brain revealed successful expression of the BDNF gene both in vitro and in vivo. The results of a survival assay of chick dorsal root ganglion cells showed that the produced BDNF was biologically active. We consider, therefore, this newly constructed recombinant adenovirus to be a useful tool to deliver BDNF to degenerating neurons and to be applicable to gene therapy of neurodegenerative diseases and nerve trauma.
|
| Volume |
212(2)
|
| Pages |
712-7
|
| Published |
1995-7-17
|
| DOI |
10.1006/bbrc.1995.2027
|
| PII |
S0006-291X(85)72027-X
|
| PMID |
7542888
|
| MeSH |
Adenoviridae / genetics*
Animals
Base Sequence
Brain / metabolism
Brain-Derived Neurotrophic Factor
Cell Survival
Chickens
Culture Media, Conditioned
DNA, Complementary / genetics
Ganglia, Spinal / cytology
Gene Expression
Gene Transfer Techniques
Genetic Vectors*
HeLa Cells
Humans
Mice
Molecular Sequence Data
Nerve Growth Factors / genetics
Nerve Tissue Proteins / genetics*
Polymerase Chain Reaction
RNA, Messenger / metabolism
RNA-Directed DNA Polymerase
Recombinant Proteins / biosynthesis
|
| IF |
2.985
|
| Times Cited |
13
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
pAxcw pAdex1cw (RDB00917) |
