Reference - Detail
|Author||Yoshizawa Y, Sato R, Tsuchihara K, Ozaki K, Mita K, Asaoka K, Taniai K.|
|Title||Ligand carrier protein genes expressed in larval chemosensory organs of Bombyx mori.|
|Journal||Insect Biochem. Mol. Biol.|
Expressed sequence tags (ESTs) of the maxillary galea of the silkworm were analyzed to identify proteins involved in food selection systems. From the 1251 redundant genes of the ESTs, we identified 7 odorant-binding protein-like genes (bmObpL), 6 takeout-like genes (bmToL), and 6 chemosensory protein genes (bmCsp). Quantitative RT-PCR analysis indicated that bmObpL1, bmObpL2, bmObpL3, bmObpL5, bmToL1, bmToL3, and bmorCsp15 were predominantly expressed in the larval oral appendages, such as the maxilla, labrum, labium and antenna. Immunocytochemical analysis indicated that the proteins of bmObpL1, bmObpL3, and bmToL1 were localized in the gustatory chemosensilla on the maxillary galea and olfactory sensilla in the antenna. The proteins encoded by bmObpL1 and bmObpL3 were detected in the gustatory chemosensilla of the epipharynx. However, bmObpL1 and bmToL1 were also detected in tactile hairs and in the epidermis of several chemosensory organs. The bmObpL2 protein was localized inside and in the epidermis around the chemosensilla, tactile hairs, and wide surface of the epipharynx. From these results, bmObpL3 is the most likely to have a dedicated role in chemoreception in the silkworm, Bombyx mori.
|MeSH||Amino Acid Sequence Animals Arthropod Antennae / cytology Arthropod Antennae / metabolism Blotting, Western Bombyx* / cytology Bombyx* / genetics Bombyx* / metabolism Chemoreceptor Cells / cytology Chemoreceptor Cells / metabolism* Cloning, Molecular Escherichia coli Expressed Sequence Tags Food Preferences / physiology Gene Expression Profiling Gene Library Genome, Insect Immunohistochemistry Insect Proteins / genetics* Insect Proteins / metabolism Larva / cytology Larva / genetics* Larva / metabolism Ligands Molecular Sequence Data Multigene Family Phylogeny Plasmids Receptors, Odorant / genetics* Receptors, Odorant / metabolism Recombinant Proteins Reverse Transcriptase Polymerase Chain Reaction Sensilla / cytology Sensilla / metabolism* Sequence Alignment|
|WOS Category||ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY|