RRC ID 12266
著者 Kodama Y, Nagaya S, Shinmyo A, Kato K.
タイトル Mapping and characterization of DNase I hypersensitive sites in Arabidopsis chromatin.
ジャーナル Plant Cell Physiol
Abstract Recent genome-wide analyses of yeast and human chromatin revealed the widespread prevalence of DNase I hypersensitive sites (DNase I HSs) at gene regulatory regions with possible roles in eukaryotic gene regulation. The presence of DNase I HSs in plants has been described for only a few genes, and we analyzed the chromatin structure of an 80 kb genomic region containing 30 variably expressed genes by DNase I sensitivity assay at 500 bp resolution in Arabidopsis. Distinct DNase I HSs were found at the 5' and/or 3' ends of most genes irrespective of their expression levels. Further analysis of well-characterized genes showed that the DNase I HSs occurred near cis-regulatory elements in the promoters of these genes. Upon transcriptional activation of a heat-inducible gene, the DNase I HS was extended into the vicinity of a cis-element and adjacent TATA element in the promoter. Concomitant with this change in DNase I HS, histones were acetylated, removed from the promoter, and a transcription activator bound to this cis-element. These results suggest that the DNase I HSs participate in the transcriptional regulation of Arabidopsis genes by enhancing the access of chromatin remodeling factors and/or transcription factors to their target sites as seen in yeast and human chromatin.
巻・号 48(3)
ページ 459-70
公開日 2007-3-1
DOI 10.1093/pcp/pcm017
PII pcm017
PMID 17283013
MeSH Acetylation Arabidopsis / genetics* Arabidopsis / metabolism* Arabidopsis Proteins / genetics Base Sequence Binding Sites / genetics Chromatin / genetics* Chromatin / metabolism* Chromosome Mapping DNA Primers / genetics DNA, Plant / genetics DNA, Plant / metabolism Deoxyribonuclease I Genes, Plant Genome, Plant Heat-Shock Proteins / genetics Histones / metabolism Promoter Regions, Genetic Transcriptional Activation
IF 4.062
引用数 28
WOS 分野 PLANT SCIENCES CELL BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 rpc00008