| RRC ID |
12266
|
| 著者 |
Kodama Y, Nagaya S, Shinmyo A, Kato K.
|
| タイトル |
Mapping and characterization of DNase I hypersensitive sites in Arabidopsis chromatin.
|
| ジャーナル |
Plant Cell Physiol
|
| Abstract |
Recent genome-wide analyses of yeast and human chromatin revealed the widespread prevalence of DNase I hypersensitive sites (DNase I HSs) at gene regulatory regions with possible roles in eukaryotic gene regulation. The presence of DNase I HSs in plants has been described for only a few genes, and we analyzed the chromatin structure of an 80 kb genomic region containing 30 variably expressed genes by DNase I sensitivity assay at 500 bp resolution in Arabidopsis. Distinct DNase I HSs were found at the 5' and/or 3' ends of most genes irrespective of their expression levels. Further analysis of well-characterized genes showed that the DNase I HSs occurred near cis-regulatory elements in the promoters of these genes. Upon transcriptional activation of a heat-inducible gene, the DNase I HS was extended into the vicinity of a cis-element and adjacent TATA element in the promoter. Concomitant with this change in DNase I HS, histones were acetylated, removed from the promoter, and a transcription activator bound to this cis-element. These results suggest that the DNase I HSs participate in the transcriptional regulation of Arabidopsis genes by enhancing the access of chromatin remodeling factors and/or transcription factors to their target sites as seen in yeast and human chromatin.
|
| 巻・号 |
48(3)
|
| ページ |
459-70
|
| 公開日 |
2007-3-1
|
| DOI |
10.1093/pcp/pcm017
|
| PII |
pcm017
|
| PMID |
17283013
|
| MeSH |
Acetylation
Arabidopsis / genetics*
Arabidopsis / metabolism*
Arabidopsis Proteins / genetics
Base Sequence
Binding Sites / genetics
Chromatin / genetics*
Chromatin / metabolism*
Chromosome Mapping
DNA Primers / genetics
DNA, Plant / genetics
DNA, Plant / metabolism
Deoxyribonuclease I
Genes, Plant
Genome, Plant
Heat-Shock Proteins / genetics
Histones / metabolism
Promoter Regions, Genetic
Transcriptional Activation
|
| IF |
4.062
|
| 引用数 |
28
|
|
WOS 分野
|
PLANT SCIENCES
CELL BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
rpc00008 |
