| RRC ID |
12298
|
| 著者 |
Sone Y, Ueta E, Sannomaru Y, Miyake N, Sone H, Otsuka Y, Kondo K, Kurata T, Suzuki E.
|
| タイトル |
Dose of 3-methylcholanthrene enhances vitamin C accumulation and mRNA expression of its transporter in the liver of ODS rats and in HepG2 cells.
|
| ジャーナル |
J Biochem Mol Toxicol
|
| Abstract |
Polycyclic aromatic hydrocarbon (PAH) compounds including 3-methylcholanthrene induce harmful reactive intermediates and reactive oxygen species. This study reports the effect of 3-methylcholanthrene on the accumulation of vitamin C and the expression of vitamin C transporters. ODS rats were given l-ascorbic acid daily and intraperitoneal injections of 10 mg 3-methylcholanthrene in total. On day 10, vitamin C concentrations and the expression of vitamin C transporter in the tissues were measured. As a result, the levels of sodium-dependent vitamin C transporter (SVCTs) 1 and the l-ascorbic acid concentration in 3-methylcholanthrene-treated livers and hepatocytes have increased significantly. However, the content of vitamin C in the urine and TBARS in the liver have not changed. These results suggest that the administration of 3-methylcholanthrene elevates the requirement for vitamin C via (SVCTs) 1 due to xenobitics-metabolizing, such as the induction of cytochrome P450 family.
|
| 巻・号 |
25(6)
|
| ページ |
369-76
|
| 公開日 |
2011-1-1
|
| DOI |
10.1002/jbt.20403
|
| PMID |
21800402
|
| MeSH |
Animals
Ascorbic Acid / administration & dosage
Ascorbic Acid / metabolism*
Ascorbic Acid / urine
Blotting, Western
Cell Proliferation / drug effects
Cytochrome P-450 CYP1A1 / genetics
Environmental Pollutants / toxicity*
Glucose Transporter Type 1 / genetics
Hep G2 Cells
Humans
Lipid Peroxidation / drug effects
Liver / drug effects*
Liver / metabolism
Male
Methylcholanthrene / toxicity*
Microsomes, Liver / drug effects
Microsomes, Liver / metabolism
RNA, Messenger / genetics*
Rats
Rats, Inbred Strains
Real-Time Polymerase Chain Reaction
Sodium-Coupled Vitamin C Transporters / genetics*
|
| IF |
3.606
|
| 引用数 |
0
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
TOXICOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Hep G2 |
