Reference - Detail
| RRC ID | 12327 |
|---|---|
| Author | Templeton GW, Nimick M, Morrice N, Campbell D, Goudreault M, Gingras AC, Takemiya A, Shimazaki K, Moorhead GB. |
| Title | Identification and characterization of AtI-2, an Arabidopsis homologue of an ancient protein phosphatase 1 (PP1) regulatory subunit. |
| Journal | Biochem J |
| Abstract |
PP1 (protein phosphatase 1) is among the most conserved enzymes known, with one or more isoforms present in all sequenced eukaryotic genomes. PP1 dephosphorylates specific serine/threonine phosphoproteins as defined by associated regulatory or targeting subunits. In the present study we performed a PP1-binding screen to find putative PP1 interactors in Arabidopsis thaliana and uncovered a homologue of the ancient PP1 interactor, I-2 (inhibitor-2). Bioinformatic analysis revealed remarkable conservation of three regions of plant I-2 that play key roles in binding to PP1 and regulating its function. The sequence-related properties of plant I-2 were compared across eukaryotes, indicating a lack of I-2 in some species and the emergence points from key motifs during the evolution of this ancient regulator. Biochemical characterization of AtI-2 (Arabidopsis I-2) revealed its ability to inhibit all plant PP1 isoforms and inhibitory dependence requiring the primary interaction motif known as RVXF. Arabidopsis I-2 was shown to be a phosphoprotein in vivo that was enriched in the nucleus. TAP (tandem affinity purification)-tag experiments with plant I-2 showed in vivo association with several Arabidopsis PP1 isoforms and identified other potential I-2 binding proteins. |
| Volume | 435(1) |
| Pages | 73-83 |
| Published | 2011-4-1 |
| DOI | 10.1042/BJ20101035 |
| PII | BJ20101035 |
| PMID | 21222654 |
| MeSH | Amino Acid Sequence Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / chemistry* Arabidopsis Proteins / genetics Arabidopsis Proteins / isolation & purification Arabidopsis Proteins / metabolism Cell Line Cell Nucleus / metabolism Computational Biology / methods Databases, Protein Molecular Sequence Data Phosphoproteins / chemistry Phosphoproteins / genetics Phosphoproteins / isolation & purification Phosphoproteins / metabolism Phylogeny Plant Epidermis / cytology Plant Epidermis / metabolism Plant Leaves / cytology Plant Leaves / metabolism Plant Structures / metabolism Protein Isoforms / genetics Protein Isoforms / isolation & purification Protein Isoforms / metabolism Protein Phosphatase 1 / chemistry* Protein Phosphatase 1 / genetics Protein Phosphatase 1 / isolation & purification Protein Phosphatase 1 / metabolism Protein Subunits / chemistry Protein Subunits / genetics Protein Subunits / isolation & purification Protein Subunits / metabolism Protein Transport Recombinant Fusion Proteins / metabolism Sequence Alignment Sequence Homology, Amino Acid |
| IF | 4.097 |
| Times Cited | 30 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pda02740 |