Reference - Detail
| RRC ID | 12341 |
|---|---|
| Author | Stoppel R, Lezhneva L, Schwenkert S, Torabi S, Felder S, Meierhoff K, Westhoff P, Meurer J. |
| Title | Recruitment of a ribosomal release factor for light- and stress-dependent regulation of petB transcript stability in Arabidopsis chloroplasts. |
| Journal | Plant Cell |
| Abstract |
Land plant genomes encode four functional ribosomal peptide chain release factors (Prf) of eubacterial origin, two (PrfA and PrfB homologs) for each endosymbiotic organelle. Formerly, we have shown that the Arabidopsis thaliana chloroplast-localized PrfB homolog, PrfB1, is required not only for termination of translation but also for stabilization of UGA stop codon-containing chloroplast transcripts. A previously undiscovered PrfB-like protein, PrfB3, is localized to the chloroplast stroma in a petB RNA-containing complex and found only in vascular plants. Highly conserved positions of introns unequivocally indicate that PrfB3 arose from a duplication of PrfB1. Notably, PrfB3 is lacking the two most important tripeptide motifs characteristic for all eubacterial and organellar PrfB homologs described so far: the stop codon recognition motif SPF and the catalytic center GGQ for peptidyl-tRNA hydrolysis. Complementation studies, as well as functional and molecular analyses of two allelic mutations in Arabidopsis, both of which lead to a specific deficiency of the cytochrome b₆f complex, revealed that PrfB3 is essentially required for photoautotrophic growth. Plastid transcript, polysome, and translation analyses indicate that PrfB3 has been recruited in vascular plants for light- and stress-dependent regulation of stability of 3' processed petB transcripts to adjust cytochrome b₆ levels. |
| Volume | 23(7) |
| Pages | 2680-95 |
| Published | 2011-7-1 |
| DOI | 10.1105/tpc.111.085324 |
| PII | tpc.111.085324 |
| PMID | 21771930 |
| PMC | PMC3226201 |
| MeSH | Amino Acid Sequence Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / classification Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Chloroplasts / genetics Chloroplasts / metabolism* Chloroplasts / ultrastructure Codon, Terminator / metabolism Cytochrome b6f Complex / genetics Cytochrome b6f Complex / metabolism Electron Transport Gene Expression Regulation, Plant* Light Molecular Sequence Data Multigene Family Mutation Phylogeny Plant Proteins / genetics Plant Proteins / metabolism* Protein Isoforms / genetics Protein Isoforms / metabolism RNA Stability* RNA-Binding Proteins / classification RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Ribosomal Proteins / classification Ribosomal Proteins / genetics Ribosomal Proteins / metabolism* Sequence Alignment Stress, Physiological |
| IF | 9.618 |
| Times Cited | 27 |
| WOS Category | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pda09666 |