| RRC ID |
12373
|
| 著者 |
Naito Y, Kajikawa H, Mizushima K, Shimozawa M, Kuroda M, Katada K, Takagi T, Handa O, Kokura S, Ichikawa H, Yoshida N, Matsui H, Yoshikawa T.
|
| タイトル |
Rebamipide, a gastro-protective drug, inhibits indomethacin-induced apoptosis in cultured rat gastric mucosal cells: association with the inhibition of growth arrest and DNA damage-induced 45 alpha expression.
|
| ジャーナル |
Dig Dis Sci
|
| Abstract |
Rebamipide, a gastromucosal protective drug, suppresses indomethacin-induced gastropathy in humans and rodents. Effects of rebamipide on gene expression in indomethacin-treated gastric mucosal cells (RGM1) were investigated using high-density oligonucleotide arrays. Indomethacin induced apoptosis in RGM1 cells in a dose-dependent manner. Rebamipide pretreatment significantly reduced indomethacin-induced apoptosis. We used gene expression profiling on high-density oligonucleotide probe arrays to characterize the transcriptional response of RGM1 cells to indomethacin treatment for 6 hr. Of the 8,799 probes examined, 717 (8.1%) were induced (400 probes) or repressed (317 probes) at least 1.5-fold. Among the 158 genes that were induced by indomethacin at least 2.0-fold, four genes that were down-regulated by rebamipide at least 2.0-fold are listed: growth arrest and DNA-damage-inducible 45 alpha (GADD 45 alpha), golgi SNAP receptor complex member 1, iodothyronine deiodinases, and transcription factor 8. Real time-PCR confirmed GADD 45 alpha expression and its inhibition by rebamipide. Inhibition of apoptosis-related genes is possibly important for the cytoprotective effect of rebamipide against indomethacin-induced gastric mucosal cell injury.
|
| 巻・号 |
50 Suppl 1
|
| ページ |
S104-12
|
| 公開日 |
2005-10-1
|
| DOI |
10.1007/s10620-005-2814-3
|
| PMID |
16184411
|
| MeSH |
Alanine / analogs & derivatives*
Alanine / pharmacology
Animals
Anti-Inflammatory Agents, Non-Steroidal / adverse effects*
Anti-Ulcer Agents / pharmacology*
Apoptosis / drug effects*
Cell Culture Techniques
Cell Cycle Proteins / biosynthesis*
Cell Cycle Proteins / genetics
DNA Damage
Dose-Response Relationship, Drug
Down-Regulation
Gastric Mucosa / drug effects*
Gastric Mucosa / pathology*
Gene Expression Profiling*
Indomethacin / adverse effects*
Nuclear Proteins / biosynthesis*
Nuclear Proteins / genetics
Oligonucleotide Probes
Polymerase Chain Reaction
Quinolones / pharmacology*
Rats
|
| IF |
2.751
|
| 引用数 |
18
|
|
WOS 分野
|
GASTROENTEROLOGY & HEPATOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RGM1(RCB0876) |
