Reference - Detail
| RRC ID | 12391 |
|---|---|
| Author | Yamamoto D, Ikeshita N, Matsubara T, Tasaki H, Herningtyas EH, Toda K, Iida K, Takahashi Y, Kaji H, Chihara K, Okimura Y. |
| Title | GHRP-2, a GHS-R agonist, directly acts on myocytes to attenuate the dexamethasone-induced expressions of muscle-specific ubiquitin ligases, Atrogin-1 and MuRF1. |
| Journal | Life Sci |
| Abstract |
Recent reports suggest that Atrogin-1 and MuRF1, E3 ubiquitin ligases, play a pivotal role in muscle atrophy. In the present study, effect of Growth Hormone Releasing Peptide-2 (GHRP-2), a GH secretagogue receptor (GHS-R) agonist, on the expressions of Atrogin-1 and MuRF1 in vivo rat muscles was examined. Dexamethasone administration increased Atrogin-1 mRNA level in rat soleus muscle. The increased mRNA level of Atrogin-1 was significantly attenuated by GHRP-2. In addition, GHRP-2 decreased MuRF1 mRNA level irrespective of the presence of dexamethasone. Although IGF-I is a well-known protective factor for muscle atrophy, GHRP-2 did not influence plasma IGF-I levels and IGF-I mRNA levels in muscles. To clarify a direct effect of GHRP-2, differentiated C2C12 myocytes were used. Ten micrometer dexamethasone increased both Atrogin-1 and MuRF1 mRNA levels in C2C12 cells. GHRP-2 attenuated dexamethasone-induced expression of them dose-dependently and decreased the basal level of MuRF1 mRNA. The suppressive effect on the expressions of Atrogin-1 and MuRF1 by GHRP-2 was blocked by [D-Lys(3)]-GHRP-6, a GHS-R1a blocker, suggesting the effect of GHRP-2 was mediated through GHS-R1a. Taken together, GHRP-2 directly attenuates Atrogin-1 and MuRF1 mRNA levels through ghrelin receptors in myocytes. |
| Volume | 82(9-10) |
| Pages | 460-6 |
| Published | 2008-2-27 |
| DOI | 10.1016/j.lfs.2007.11.019 |
| PII | S0024-3205(07)00851-X |
| PMID | 18191156 |
| MeSH | Animals Cell Line Dexamethasone / pharmacology* Dose-Response Relationship, Drug Gene Expression / drug effects Glucocorticoids / pharmacology Male Mice Muscle Cells / cytology Muscle Cells / drug effects* Muscle Cells / metabolism Muscle Proteins / genetics* Oligopeptides / pharmacology* RNA, Messenger / genetics RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Receptors, Ghrelin / agonists Receptors, Ghrelin / genetics Reverse Transcriptase Polymerase Chain Reaction SKP Cullin F-Box Protein Ligases / genetics Tripartite Motif Proteins Ubiquitin-Protein Ligases / genetics* |
| IF | 3.647 |
| Times Cited | 22 |
| WOS Category | MEDICINE, RESEARCH & EXPERIMENTAL PHARMACOLOGY & PHARMACY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | C2C12(RCB0987) |