| RRC ID |
1242
|
| Author |
Adachi K, Osaki M, Kase S, Takeda A, Ito H.
|
| Title |
Anti-Fas antibody-induced apoptosis and its signal transduction in human gastric carcinoma cell lines.
|
| Journal |
Int J Oncol
|
| Abstract |
The Fas-Fas ligand system is one of the factors involved in cell death signaling. Aberrations in the signaling pathways leading to Fas-mediated apoptosis in tumor cells have been reported in a variety of human malignant tumors. However, the Fas-mediated apoptotic pathway has not been sufficiently elucidated in human gastric carcinomas. We examined the apoptotic pathway induced by anti-Fas antibody using seven human gastric carcinoma cell lines. Apoptosis was induced in a delayed fashion and the apoptotic indices (AI) after 48 h were approximately 30-40% in MKN-45 and KATO-III cells, which both showed cleavage of the Bid protein and release of Cytochrome c from the mitochondria. Our data also demonstrated no significant relationship between the expressions of various apoptosis-related proteins and the sensitivity or resistance to anti-Fas antibody-induced apoptosis, as far as we examined. Furthermore, the apoptosis signal was inhibited by treatment with Caspase-9 and -3 inhibitors in MKN-45 and KATO-III. These findings suggest that anti-Fas antibody induced apoptosis through the type II signaling pathway in the human gastric carcinoma cell lines, MKN-45 and KATO-III.
|
| Volume |
23(3)
|
| Pages |
713-9
|
| Published |
2003-9-1
|
| PMID |
12888908
|
| MeSH |
Apoptosis*
Blotting, Western
Carcinoma / metabolism*
Carcinoma / pathology*
Caspase 3
Caspase 9
Caspase Inhibitors
Caspases / metabolism
Cell Line
Cell Line, Tumor
Cell Survival
Cytochromes c / metabolism
Enzyme Inhibitors / pharmacology
Humans
Signal Transduction*
Stomach Neoplasms / metabolism*
Stomach Neoplasms / pathology*
Time Factors
fas Receptor / chemistry
fas Receptor / immunology*
|
| IF |
3.899
|
| Times Cited |
4
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
MKN7(RCB0999)
MKN45(RCB1001)
MKN74(RCB1002) |
