論文 - 詳細
| RRC ID | 12463 |
|---|---|
| 著者 | Herring M, Davenport N, Stephan K, Campbell S, White R, Kark J, Wolkow TD. |
| タイトル | Fission yeast Rad26ATRIP delays spindle-pole-body separation following interphase microtubule damage. |
| ジャーナル | J Cell Sci |
| Abstract |
The conserved fission yeast protein Rad26(ATRIP) preserves genomic stability by occupying central positions within DNA-structure checkpoint pathways. It is also required for proper cellular morphology, chromosome stability and following treatment with microtubule poisons. Here, we report that mutation of a putative nuclear export sequence in Rad26(ATRIP) disrupted its cytoplasmic localization in untreated cells and conferred abnormal cellular morphology, minichromosome instability and sensitivity to microtubule poisons without affecting DNA-structure checkpoint signaling. This mutation also disrupted a delay to spindle-pole-body separation that occurred following microtubule damage in G(2). Together, these results demonstrate that Rad26(ATRIP) participates in two genetically defined checkpoint pathways--one that responds to genomic damage and the other to microtubule damage. This response to microtubule damage delays spindle-pole-body separation and, in doing so, might preserve both cellular morphology and chromosome stability. |
| 巻・号 | 123(Pt 9) |
| ページ | 1537-45 |
| 公開日 | 2010-5-1 |
| DOI | 10.1242/jcs.049478 |
| PII | jcs.049478 |
| PMID | 20375067 |
| MeSH | Alleles Amino Acid Sequence Benzimidazoles / pharmacology Carbamates / pharmacology Cell Cycle Proteins / chemistry Cell Cycle Proteins / metabolism* Chromosomal Instability / drug effects DNA Damage* DNA, Fungal / metabolism G2 Phase / drug effects Interphase* / drug effects Mad2 Proteins Microtubules / metabolism* Mitosis / drug effects Molecular Sequence Data Nuclear Export Signals Nuclear Proteins / metabolism Recombinant Fusion Proteins / metabolism Schizosaccharomyces / cytology Schizosaccharomyces / drug effects Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / metabolism* Signal Transduction / drug effects Spindle Apparatus / drug effects Spindle Apparatus / metabolism* |
| IF | 4.573 |
| 引用数 | 1 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| 酵母 | |