RRC ID 12473
Author Takahashi K, Imano R, Kibe T, Seimiya H, Muramatsu Y, Kawabata N, Tanaka G, Matsumoto Y, Hiromoto T, Koizumi Y, Nakazawa N, Yanagida M, Yukawa M, Tsuchiya E, Ueno M.
Title Fission yeast Pot1 and RecQ helicase are required for efficient chromosome segregation.
Journal Mol Cell Biol
Abstract Pot1 is a single-stranded telomere-binding protein that is conserved from fission yeast to mammals. Deletion of Schizosaccharomyces pombe pot1(+) causes immediate telomere loss. S. pombe Rqh1 is a homolog of the human RecQ helicase WRN, which plays essential roles in the maintenance of genomic stability. Here, we demonstrate that a pot1Δ rqh1-hd (helicase-dead) double mutant maintains telomeres that are dependent on Rad51-mediated homologous recombination. Interestingly, the pot1Δ rqh1-hd double mutant displays a "cut" (cell untimely torn) phenotype and is sensitive to the antimicrotubule drug thiabendazole (TBZ). Moreover, the chromosome ends of the double mutant do not enter the pulsed-field electrophoresis gel. These results suggest that the entangled chromosome ends in the pot1Δ rqh1-hd double mutant inhibit chromosome segregation, signifying that Pot1 and Rqh1 are required for efficient chromosome segregation. We also found that POT1 knockdown, WRN-deficient human cells are sensitive to the antimicrotubule drug vinblastine, implying that some of the functions of S. pombe Pot1 and Rqh1 may be conserved in their respective human counterparts POT1 and WRN.
Volume 31(3)
Pages 495-506
Published 2011-2-1
DOI 10.1128/MCB.00613-10
PII MCB.00613-10
PMID 21098121
PMC PMC3028624
MeSH Chromosome Segregation* / drug effects Chromosomes, Fungal / metabolism* DNA Helicases / metabolism* Exodeoxyribonucleases / metabolism Gene Silencing / drug effects HeLa Cells Humans Microbial Viability / drug effects Mitosis / drug effects Mutation / genetics RecQ Helicases / metabolism Recombination, Genetic / drug effects Replication Protein A / metabolism Schizosaccharomyces / cytology Schizosaccharomyces / drug effects Schizosaccharomyces / enzymology* Schizosaccharomyces / genetics* Schizosaccharomyces pombe Proteins / metabolism* Shelterin Complex Telomere / metabolism Telomere-Binding Proteins / metabolism* Thiabendazole / pharmacology Vinblastine / pharmacology Werner Syndrome Helicase
IF 3.611
Times Cited 10
Yeast FY7512(JY741)