論文 - 詳細
| RRC ID | 12505 |
|---|---|
| 著者 | Onishi M, Koga T, Hirata A, Nakamura T, Asakawa H, Shimoda C, Bähler J, Wu JQ, Takegawa K, Tachikawa H, Pringle JR, Fukui Y. |
| タイトル | Role of septins in the orientation of forespore membrane extension during sporulation in fission yeast. |
| ジャーナル | Mol Cell Biol |
| Abstract |
During yeast sporulation, a forespore membrane (FSM) initiates at each spindle-pole body and extends to form the spore envelope. We used Schizosaccharomyces pombe to investigate the role of septins during this process. During the prior conjugation of haploid cells, the four vegetatively expressed septins (Spn1, Spn2, Spn3, and Spn4) coassemble at the fusion site and are necessary for its normal morphogenesis. Sporulation involves a different set of four septins (Spn2, Spn5, Spn6, and the atypical Spn7) that does not include the core subunits of the vegetative septin complex. The four sporulation septins form a complex in vitro and colocalize interdependently to a ring-shaped structure along each FSM, and septin mutations result in disoriented FSM extension. The septins and the leading-edge proteins appear to function in parallel to orient FSM extension. Spn2 and Spn7 bind to phosphatidylinositol 4-phosphate [PtdIns(4)P] in vitro, and PtdIns(4)P is enriched in the FSMs, suggesting that septins bind to the FSMs via this lipid. Cells expressing a mutant Spn2 protein unable to bind PtdIns(4)P still form extended septin structures, but these structures fail to associate with the FSMs, which are frequently disoriented. Thus, septins appear to form a scaffold that helps to guide the oriented extension of the FSM. |
| 巻・号 | 30(8) |
| ページ | 2057-74 |
| 公開日 | 2010-4-1 |
| DOI | 10.1128/MCB.01529-09 |
| PII | MCB.01529-09 |
| PMID | 20123972 |
| PMC | PMC2849465 |
| MeSH | Amino Acid Sequence Cell Membrane / metabolism* Cell Membrane / ultrastructure Cell Surface Extensions / metabolism* Cell Surface Extensions / ultrastructure GTP-Binding Proteins / genetics GTP-Binding Proteins / metabolism* Molecular Sequence Data Mutation Phosphatidylinositol Phosphates / metabolism Schizosaccharomyces* / cytology Schizosaccharomyces* / physiology Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Sequence Alignment Spores, Fungal* / physiology Spores, Fungal* / ultrastructure |
| IF | 3.611 |
| 引用数 | 25 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | (968 972 975 JB61 JB68 JW193 SY13)? |