RRC ID 12507
著者 Nakamura T, Nakamura-Kubo M, Nakamura T, Shimoda C.
タイトル Novel fission yeast Cdc7-Dbf4-like kinase complex required for the initiation and progression of meiotic second division.
ジャーナル Mol Cell Biol
Abstract Cdc7, a conserved serine/threonine protein kinase, controls initiation of DNA replication. A regulatory subunit, Dbf4, stimulates the kinase activity of Cdc7 and recruits it to the replication origins. Schizosaccharomyces pombe has a homologous kinase complex, composed of Hsk1 and Dfp1/Him1. Here, we report a novel protein kinase of S. pombe, Spo4, which shares common structural features with the Cdc7 kinases. In spite of the structural similarities, Spo4 is dispensable for mitotic growth and premeiotic DNA replication. Intriguingly, spo4 null mutants are defective in initiation and progression of the second meiotic division. Spindles for meiosis II are often fragmented. Spo4 kinase activity is markedly enhanced when the enzyme is associated with its regulatory subunit, Spo6, a Dbf4-like protein. Expression of Spo4 is specifically induced during meiosis. Spo4 is preferentially present in nuclei, but this nuclear localization does not require Spo6. These results suggest that Spo4 is a Cdc7 kinase whose primary role is in meiosis, not in DNA replication. This is the first report of an organism which has two Cdc7-related kinase complexes with different biological functions.
巻・号 22(1)
ページ 309-20
公開日 2002-1-1
DOI 10.1128/MCB.22.1.309-320.2002
PMID 11739743
PMC PMC134210
MeSH Amino Acid Sequence Animals Base Sequence Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Nucleus / metabolism Cell Separation DNA / metabolism DNA-Binding Proteins / metabolism Flow Cytometry Fungal Proteins / genetics Fungal Proteins / metabolism* Genes, Fungal Humans Macromolecular Substances Meiosis / physiology* Microscopy, Fluorescence Microtubules / metabolism Molecular Sequence Data Nuclear Proteins / genetics Nuclear Proteins / isolation & purification Nuclear Proteins / metabolism Phosphoproteins / genetics Phosphoproteins / metabolism Protein Serine-Threonine Kinases / chemistry Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / isolation & purification Protein Serine-Threonine Kinases / metabolism* RNA-Binding Proteins Recombinant Fusion Proteins / metabolism Saccharomyces cerevisiae Proteins* Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / isolation & purification Schizosaccharomyces pombe Proteins / metabolism* Two-Hybrid System Techniques
IF 3.611
引用数 42
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
酵母 TN29 TN47 TN53 FY7195(HA46-11B) FY7051(JZ670) FY7476(AB4)