RRC ID 12533
Author Kerres A, Jakopec V, Fleig U.
Title The conserved Spc7 protein is required for spindle integrity and links kinetochore complexes in fission yeast.
Journal Mol. Biol. Cell
Abstract Spc7, a member of the conserved Spc105/KNL-1 family of kinetochore proteins, was identified as an interaction partner of the EB1 homologue Mal3. Spc7 associates with the central centromere region of the chromosome but does not affect transcriptional silencing. Here, we show that Spc7 is required for the integrity of the spindle as well as for targeting of MIND but not of Ndc80 complex components to the kinetochore. Spindle defects in spc7 mutants were severe ranging from the inability to form a bipolar spindle in early mitosis to broken spindles in midanaphase B. spc7 mutant phenotypes were partially rescued by extra alpha-tubulin or extra Mal2. Thus, Spc7 interacts genetically with the Mal2-containing Sim4 complex.
Volume 18(7)
Pages 2441-54
Published 2007-7
DOI 10.1091/mbc.e06-08-0738
PII E06-08-0738
PMID 17442892
PMC PMC1924829
MeSH Amino Acid Sequence Cell Polarity Chromosomal Proteins, Non-Histone / metabolism* Chromosome Segregation Chromosomes, Fungal / metabolism Conserved Sequence* Gene Silencing Genes, Fungal Kinetochores / metabolism* Mitosis Multiprotein Complexes Mutation / genetics Schizosaccharomyces / cytology Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / metabolism* Spindle Apparatus / metabolism* Temperature Transcription, Genetic
IF 3.905
Times Cited 22
Yeast FY15673(ANF251-9A)