RRC ID 12541
Author Hayashi T, Fujita Y, Iwasaki O, Adachi Y, Takahashi K, Yanagida M.
Title Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres.
Journal Cell
Abstract Centromeres contain specialized chromatin that includes the centromere-specific histone H3 variant, spCENP-A/Cnp1. Here we report identification of five fission yeast centromere proteins, Mis14-18. Mis14 is recruited to kinetochores independently of CENP-A, and, conversely, CENP-A does not require Mis14 to associate with centromeres. In contrast, Mis15, Mis16 (strong similarity with human RbAp48 and RbAp46), Mis17, and Mis18 are all part of the CENP-A recruitment pathway. Mis15 and Mis17 form an evolutionarily conserved complex that also includes Mis6. Mis16 and Mis18 form a complex and maintain the deacetylated state of histones specifically in the central core of centromeres. Mis16 and Mis18 are the most upstream factors in kinetochore assembly as they can associate with kinetochores in all kinetochore mutants except for mis18 and mis16, respectively. RNAi knockdown in human cells shows that Mis16 function is conserved as RbAp48 and RbAp46 are both required for localization of human CENP-A.
Volume 118(6)
Pages 715-29
Published 2004-9-17
DOI 10.1016/j.cell.2004.09.002
PII S0092867404008323
PMID 15369671
MeSH Acetylation Carrier Proteins / genetics Carrier Proteins / isolation & purification Carrier Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Survival / genetics Centromere / genetics Centromere / metabolism* Centromere / ultrastructure Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / metabolism* Chromosome Segregation / genetics HeLa Cells Histones / genetics Histones / metabolism* Humans Kinetochores / metabolism Mutation / genetics Nuclear Proteins / genetics Nuclear Proteins / isolation & purification Nuclear Proteins / metabolism* RNA Interference Retinoblastoma-Binding Protein 4 Retinoblastoma-Binding Protein 7 Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces / ultrastructure Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / isolation & purification Schizosaccharomyces pombe Proteins / metabolism*
IF 36.216
Times Cited 242
Yeast genomic library