Reference - Detail
| RRC ID | 12572 |
|---|---|
| Author | Rodríguez-Sánchez L, Rodríguez-López M, García Z, Tenorio-Gómez M, Schvartzman JB, Krimer DB, Hernández P. |
| Title | The fission yeast rDNA-binding protein Reb1 regulates G1 phase under nutritional stress. |
| Journal | J Cell Sci |
| Abstract |
Yeast Reb1 and its mammalian ortholog TTF1 are conserved Myb-type DNA-binding proteins that bind to specific sites near the 3'-end of rRNA genes (rDNA). Here, they participate in the termination of transcription driven by RNA polymerase I and block DNA replication forks approaching in the opposite direction. We found that Schizosaccharomyces pombe Reb1 also upregulates transcription of the ste9(+) gene that is required for nitrogen-starvation-induced growth arrest with a G1 DNA content and sexual differentiation. Ste9 activates the anaphase-promoting complex or cyclosome ('APC/C') in G1, targeting B-cyclin for proteasomal degradation in response to nutritional stress. Reb1 binds in vivo and in vitro to a specific DNA sequence at the promoter of ste9(+), similar to the sequence recognized in the rDNA, and this binding is required for ste9(+) transcriptional activation and G1 arrest. This suggests that Reb1 acts as a link between rDNA metabolism and cell cycle control in response to nutritional stress. In agreement with this new role for Reb1 in the regulation of the G1-S transition, reb1Δ and wee1(ts) mutations are synthetically lethal owing to the inability of these cells to lengthen G1 before entering S phase. Similarly, reb1Δ cdc10(ts) cells are unable to arrest in G1 and die at the semi-permissive temperature. |
| Volume | 124(Pt 1) |
| Pages | 25-34 |
| Published | 2011-1-1 |
| DOI | 10.1242/jcs.070987 |
| PII | jcs.070987 |
| PMID | 21118960 |
| MeSH | Base Sequence DNA, Ribosomal / genetics DNA, Ribosomal / metabolism* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* G1 Phase* Gene Expression Regulation, Fungal* Molecular Sequence Data Nitrogen / deficiency Protein Binding Schizosaccharomyces / cytology Schizosaccharomyces / genetics Schizosaccharomyces / growth & development Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Stress, Physiological Transcription Factors / genetics Transcription Factors / metabolism* |
| IF | 4.573 |
| Times Cited | 16 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Yeast | |