論文 - 詳細
| RRC ID | 12593 |
|---|---|
| 著者 | Dunleavy EM, Pidoux AL, Monet M, Bonilla C, Richardson W, Hamilton GL, Ekwall K, McLaughlin PJ, Allshire RC. |
| タイトル | A NASP (N1/N2)-related protein, Sim3, binds CENP-A and is required for its deposition at fission yeast centromeres. |
| ジャーナル | Mol Cell |
| Abstract |
A defining feature of centromeres is the presence of the histone H3 variant CENP-A(Cnp1). It is not known how CENP-A(Cnp1) is specifically delivered to, and assembled into, centromeric chromatin. Through a screen for factors involved in kinetochore integrity in fission yeast, we identified Sim3. Sim3 is homologous to known histone binding proteins NASP(Human) and N1/N2(Xenopus) and aligns with Hif1(S. cerevisiae), defining the SHNi-TPR family. Sim3 is distributed throughout the nucleoplasm, yet it associates with CENP-A(Cnp1) and also binds H3. Cells defective in Sim3 function have reduced levels of CENP-A(Cnp1) at centromeres (and increased H3) and display chromosome segregation defects. Sim3 is required to allow newly synthesized CENP-A(Cnp1) to accumulate at centromeres in S and G2 phase-arrested cells in a replication-independent mechanism. We propose that one function of Sim3 is to act as an escort that hands off CENP-A(Cnp1) to chromatin assembly factors, allowing its incorporation into centromeric chromatin. |
| 巻・号 | 28(6) |
| ページ | 1029-44 |
| 公開日 | 2007-12-28 |
| DOI | 10.1016/j.molcel.2007.10.010 |
| PII | S1097-2765(07)00692-2 |
| PMID | 18158900 |
| PMC | PMC2193228 |
| MeSH | Amino Acid Motifs Amino Acid Sequence Animals Autoantigens / genetics Autoantigens / metabolism* Binding Sites Blotting, Western Centromere / metabolism* Centromere Protein A Chromatin Immunoprecipitation Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / metabolism* Fungal Proteins / genetics Fungal Proteins / metabolism* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Humans Microscopy, Fluorescence Molecular Sequence Data Mutation Nuclear Proteins / genetics Nuclear Proteins / metabolism* Protein Binding Protein Structure, Tertiary Protein Transport Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Schizosaccharomyces / chemistry Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Sequence Homology, Amino Acid Xenopus |
| IF | 15.584 |
| 引用数 | 73 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY8481 |