| RRC ID |
12593
|
| 著者 |
Dunleavy EM, Pidoux AL, Monet M, Bonilla C, Richardson W, Hamilton GL, Ekwall K, McLaughlin PJ, Allshire RC.
|
| タイトル |
A NASP (N1/N2)-related protein, Sim3, binds CENP-A and is required for its deposition at fission yeast centromeres.
|
| ジャーナル |
Mol Cell
|
| Abstract |
A defining feature of centromeres is the presence of the histone H3 variant CENP-A(Cnp1). It is not known how CENP-A(Cnp1) is specifically delivered to, and assembled into, centromeric chromatin. Through a screen for factors involved in kinetochore integrity in fission yeast, we identified Sim3. Sim3 is homologous to known histone binding proteins NASP(Human) and N1/N2(Xenopus) and aligns with Hif1(S. cerevisiae), defining the SHNi-TPR family. Sim3 is distributed throughout the nucleoplasm, yet it associates with CENP-A(Cnp1) and also binds H3. Cells defective in Sim3 function have reduced levels of CENP-A(Cnp1) at centromeres (and increased H3) and display chromosome segregation defects. Sim3 is required to allow newly synthesized CENP-A(Cnp1) to accumulate at centromeres in S and G2 phase-arrested cells in a replication-independent mechanism. We propose that one function of Sim3 is to act as an escort that hands off CENP-A(Cnp1) to chromatin assembly factors, allowing its incorporation into centromeric chromatin.
|
| 巻・号 |
28(6)
|
| ページ |
1029-44
|
| 公開日 |
2007-12-28
|
| DOI |
10.1016/j.molcel.2007.10.010
|
| PII |
S1097-2765(07)00692-2
|
| PMID |
18158900
|
| PMC |
PMC2193228
|
| MeSH |
Amino Acid Motifs
Amino Acid Sequence
Animals
Autoantigens / genetics
Autoantigens / metabolism*
Binding Sites
Blotting, Western
Centromere / metabolism*
Centromere Protein A
Chromatin Immunoprecipitation
Chromosomal Proteins, Non-Histone / genetics
Chromosomal Proteins, Non-Histone / metabolism*
Fungal Proteins / genetics
Fungal Proteins / metabolism*
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Humans
Microscopy, Fluorescence
Molecular Sequence Data
Mutation
Nuclear Proteins / genetics
Nuclear Proteins / metabolism*
Protein Binding
Protein Structure, Tertiary
Protein Transport
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Schizosaccharomyces / chemistry
Schizosaccharomyces / genetics
Schizosaccharomyces / metabolism*
Schizosaccharomyces pombe Proteins / genetics
Schizosaccharomyces pombe Proteins / metabolism*
Sequence Homology, Amino Acid
Xenopus
|
| IF |
15.584
|
| 引用数 |
73
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 酵母 |
FY8481 |
