論文 - 詳細
| RRC ID | 12611 |
|---|---|
| 著者 | Onda Y, Nagamine A, Sakurai M, Kumamaru T, Ogawa M, Kawagoe Y. |
| タイトル | Distinct roles of protein disulfide isomerase and P5 sulfhydryl oxidoreductases in multiple pathways for oxidation of structurally diverse storage proteins in rice. |
| ジャーナル | Plant Cell |
| Abstract |
In the rice (Oryza sativa) endosperm, storage proteins are synthesized on the rough endoplasmic reticulum (ER), in which prolamins are sorted to protein bodies (PBs) called type-I PB (PB-I). Protein disulfide isomerase (PDI) family oxidoreductase PDIL2;3, an ortholog of human P5, contains a conserved structural disulfide in the redox-inactive thioredoxin-like (TRX) domain and was efficiently targeted to the surface of PB-I in a redox active site-dependent manner, whereas PDIL1;1, an ortholog of human PDI, was localized in the ER lumen. Complementation analyses using PDIL1;1 knockout esp2 mutant indicated that the a and a' TRX domains of PDIL1;1 exhibited similar redox activities and that PDIL2;3 was unable to perform the PDIL1;1 functions. PDIL2;3 knockdown inhibited the accumulation of Cys-rich 10-kD prolamin (crP10) in the core of PB-I. Conversely, crP10 knockdown dispersed PDIL2;3 into the ER lumen. Glutathione S-transferase-PDIL2;3 formed a stable tetramer when it was expressed in Escherichia coli, and the recombinant PDIL2;3 tetramer facilitated α-globulin(C79F) mutant protein to form nonnative intermolecular disulfide bonds in vitro. These results indicate that PDIL2;3 and PDIL1;1 are not functionally redundant in sulfhydryl oxidations of structurally diverse storage proteins and play distinct roles in PB development. We discuss PDIL2;3-dependent and PDIL2;3-independent oxidation pathways that sustain disulfide bonds of crP10 in PB-I. |
| 巻・号 | 23(1) |
| ページ | 210-23 |
| 公開日 | 2011-1-1 |
| DOI | 10.1105/tpc.110.079509 |
| PII | tpc.110.079509 |
| PMID | 21278127 |
| PMC | PMC3051231 |
| MeSH | Endoplasmic Reticulum / metabolism Endosperm / metabolism Gene Knockdown Techniques Gene Knockout Techniques Genetic Complementation Test Mutation Oryza / enzymology* Oryza / genetics Oxidation-Reduction Oxidoreductases / metabolism* Protein Disulfide-Isomerases / metabolism* Protein Folding Seed Storage Proteins / metabolism* |
| IF | 9.618 |
| 引用数 | 51 |
| WOS 分野 | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| イネ | Induced Mutation Lines |