| RRC ID |
12615
|
| Author |
Qu LQ, Wei XL, Satoh H, Kumamaru T, Ogawa M, Takaiwa F.
|
| Title |
Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene.
|
| Journal |
Theor Appl Genet
|
| Abstract |
The rice ( Oryza sativa L.) mutant of glu4a, lacking the glutelin alpha-2 subunit while the alpha-1 subunit increased (alpha-1H/alpha-2L), was used in this study. Two-dimensional electrophoresis analysis revealed that the mutant lacked the polypeptide pI6.71/alpha-2 encoded by glu4 while forming a new polypeptide of pI6.50/alpha-1. Experiments were conducted to identify the relationships between the mutated polypeptides of the mutant and to illustrate the mutation mechanism of the allele. Peptide mapping and amino-acid sequence analyses revealed that the newly formed glu4a encoded polypeptide pI6.50/alpha-1 of high homology with the deleted pI6.71/alpha-2 polypeptide which was encoded by glu4 (GluA-1). The nucleotide sequence revealed that the iso-electric point variation of the pI6.50/alpha-1 polypeptide was caused by a point mutation with nucleotide replacement at the variable region of the gene. These results suggested the possibility of altering glutelin quality by using single gene mutation.
|
| Volume |
107(1)
|
| Pages |
20-5
|
| Published |
2003-6-1
|
| DOI |
10.1007/s00122-003-1228-x
|
| PMID |
12835929
|
| MeSH |
Alleles
Amino Acid Sequence
Base Sequence
DNA, Plant / genetics
Electrophoresis, Gel, Two-Dimensional
Glutens / genetics*
Glutens / metabolism
Isoelectric Point
Molecular Sequence Data
Oryza / genetics*
Oryza / metabolism
Peptide Mapping
Point Mutation
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
|
| IF |
4.439
|
| Times Cited |
7
|
|
WOS Category
|
AGRONOMY
HORTICULTURE
PLANT SCIENCES
GENETICS & HEREDITY
|
| Resource |
| Rice |
Induced Mutation Lines |
