RRC ID 12626
Author Ogawa M, Yamaji R, Mitani T, Murata Y, Nakao M, Harada N, Nakano Y, Inui H.
Title A yeast bioassay for androgenic and anti-androgenic compounds based on the NH2- and COOH-terminal interaction of androgen receptor.
Journal Biosci. Biotechnol. Biochem.
Abstract Androgenic compounds induce an interaction between the NH(2)- and COOH-terminal regions (N-C interaction) of androgen receptor (AR). We describe a rapid yeast bioassay for androgenic and anti-androgenic compounds based on androgen-dependent β-catenin-enhanced N-C interaction. The bioassay was also effective at detecting compounds that inhibit the N-C interaction in ways that do not involve binding to the ligand-binding domain.
Volume 74(9)
Pages 1965-8
Published 2010
DOI 10.1271/bbb.100323
PII JST.JSTAGE/bbb/100323
PMID 20834141
MeSH Androgen Antagonists / analysis* Androgens / analysis* Animals Drug Evaluation, Preclinical / methods* Humans Receptors, Androgen / chemistry* Receptors, Androgen / metabolism Yeasts beta Catenin
IF 1.255
Times Cited 1
WOS Category FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Yeast YPH499?