| RRC ID |
12672
|
| 著者 |
Sasado T, Yasuoka A, Abe K, Mitani H, Furutani-Seiki M, Tanaka M, Kondoh H.
|
| タイトル |
Distinct contributions of CXCR4b and CXCR7/RDC1 receptor systems in regulation of PGC migration revealed by medaka mutants kazura and yanagi.
|
| ジャーナル |
Dev Biol
|
| Abstract |
Migratory pathways of PGCs to the gonad vary depending on the vertebrate species, yet the underlying regulatory mechanisms guiding PGCs are believed to be largely common. In teleost medaka embryo, PGC migration follows two major steps before colonizing in gonadal areas: (1) bilateral lineup in the trunk and (2) posterior drift of PGCs. kazura (kaz) and yanagi (yan) mutants of medaka isolated in mutagenesis screening were defective in the first and second steps, respectively. kaz(j2-15D) was identified as a missense mutation in chemokine receptor gene cxcr4b expressed in PGCs. Embryonic injection of cxcr4b mRNA with vasa 3' UTR rescued the PGC phenotype of kaz mutant, indicating a cell-autonomous function of cxcr4b in PGCs. yan(j6-29C) was identified as a nonsense mutation in the cxcr7/rdc1 gene encoding another chemokine receptor. cxcr7 transgene with genomic flanking sequences rescued the yan mutant phenotype efficiently at the G0 generation. cxcr7 was expressed in somites rather than PGCs. cxcr7-expressing somitic domain expanded posteriorly with its margin immediately anterior of posteriorly drifting PGCs, as if PGCs were thrusted toward the gonadal area. kaz and yan mutants are also defective in lateral line positioning, suggesting combined employment of these receptor systems in various cell migratory processes.
|
| 巻・号 |
320(2)
|
| ページ |
328-39
|
| 公開日 |
2008-8-15
|
| DOI |
10.1016/j.ydbio.2008.05.544
|
| PII |
S0012-1606(08)00909-3
|
| PMID |
18602095
|
| MeSH |
Animals
Cell Movement*
Embryo, Nonmammalian
Fish Proteins / physiology
Germ Cells / cytology*
Gonads / cytology
Mutation
Oryzias*
Receptors, CXCR / genetics
Receptors, CXCR / physiology*
Receptors, CXCR4 / genetics
Receptors, CXCR4 / physiology*
Receptors, G-Protein-Coupled / physiology*
|
| IF |
2.896
|
| 引用数 |
33
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| メダカ |
kazura^j2-15D (MT385)
yanagi^j6-29C (MT443) |
