| RRC ID |
12679
|
| Author |
Iwaki T, Osawa F, Onishi M, Koga T, Fujita Y, Hosomi A, Tanaka N, Fukui Y, Takegawa K.
|
| Title |
Characterization of vps33+, a gene required for vacuolar biogenesis and protein sorting in Schizosaccharomyces pombe.
|
| Journal |
Yeast
|
| Abstract |
From the fission yeast Schizosaccharomyces pombe we have identified and deleted vps33, a gene encoding a homologue of VPS33, which is required for vacuolar biogenesis in S. cerevisiae cells. When the vps33(+) gene is disrupted, Sz. pombe strains are temperature-sensitive for growth and contain numerous small vesicular structures stained with FM4-64 in the cells. Deletion of the Sz. pombe vps33(+) gene results in pleiotropic phenotypes consistent with the absence of normal vacuoles, including missorting of vacuolar carboxypeptidase Y, various ion- and drug-sensitivities, and sporulation defects. These results are consistent with Vps33p being necessary for the morphogenesis of vacuoles and subsequent expression of vacuolar functions in Sz. pombe cells.
|
| Volume |
20(10)
|
| Pages |
845-55
|
| Published |
2003-7-30
|
| DOI |
10.1002/yea.1011
|
| PMID |
12868054
|
| MeSH |
Amino Acid Sequence
Blotting, Western
Carboxypeptidases / genetics
Carboxypeptidases / metabolism
Cathepsin A
Genes, Fungal / genetics
Genes, Fungal / physiology*
Genetic Complementation Test
Microscopy, Fluorescence
Molecular Sequence Data
Mutagenesis, Insertional
Protein Transport / genetics
Protein Transport / physiology
Pyridinium Compounds / metabolism
Quaternary Ammonium Compounds / metabolism
Schizosaccharomyces / genetics*
Schizosaccharomyces / metabolism
Schizosaccharomyces / ultrastructure
Schizosaccharomyces pombe Proteins / genetics
Schizosaccharomyces pombe Proteins / metabolism*
Sequence Homology, Amino Acid
Vacuoles / genetics*
Vacuoles / metabolism
|
| IF |
3.143
|
| Times Cited |
32
|
|
WOS Category
|
MYCOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Yeast |
strains and plasmids |
