RRC ID 1269
Author Kawamura T, Koka R, Ma A, Kumar V.
Title Differential roles for IL-15R alpha-chain in NK cell development and Ly-49 induction.
Journal J Immunol
Abstract IL-15Ralpha-deficient (IL-15Ralpha(-/-)) mice lack NK cells. However, when bone marrow (BM) progenitors from IL-15Ralpha(-/-) mice were cultured with IL-7, stem cell factor and flt3 ligand, followed by IL-15, they were able to differentiate into functional NK cells, indicating that IL-15Ralpha is not critical for NK cell development. Whereas NK cells generated in vitro from IL-15Ralpha(-/-) BM progenitors expressed CD94/NKG2, they failed to express Ly-49 receptors. In keeping with this, when IL-15Ralpha(-/-) BM cells were transferred into wild type recipients, they gave rise to NK cells in vivo, but with greatly reduced expression of Ly-49 receptors. Furthermore, the small numbers of NK cells found in IL-15(-/-) as well as IL-15Ralpha(-/-) but not flt3 ligand(-/-) mice expressed much lower levels of Ly-49 receptors than those from wild type mice. These results indicate a novel role for IL-15Ralpha-chain in Ly-49 induction on developing NK cells.
Volume 171(10)
Pages 5085-90
Published 2003-11-15
DOI 10.4049/jimmunol.171.10.5085
PMID 14607906
MeSH Animals Antigens, Ly / biosynthesis* Antigens, Ly / metabolism Bone Marrow Cells / cytology Bone Marrow Cells / immunology Bone Marrow Cells / metabolism Bone Marrow Transplantation / immunology Cell Differentiation / genetics Cell Differentiation / immunology Cells, Cultured Down-Regulation / genetics Down-Regulation / immunology Hematopoietic Stem Cells / cytology Hematopoietic Stem Cells / immunology Hematopoietic Stem Cells / metabolism Interleukin-15 / deficiency Interleukin-15 / genetics Interleukin-15 / metabolism* Killer Cells, Natural / cytology* Killer Cells, Natural / immunology* Killer Cells, Natural / metabolism Lectins, C-Type Mice Mice, Inbred C57BL Mice, Knockout Protein Subunits / biosynthesis Protein Subunits / deficiency Protein Subunits / genetics Protein Subunits / physiology* Radiation Chimera / genetics Radiation Chimera / immunology Receptors, Immunologic / antagonists & inhibitors Receptors, Immunologic / biosynthesis Receptors, Interleukin-15 Receptors, Interleukin-2 / biosynthesis Receptors, Interleukin-2 / deficiency Receptors, Interleukin-2 / genetics Receptors, Interleukin-2 / physiology* Receptors, NK Cell Lectin-Like
IF 4.718
Times Cited 43
Human and Animal Cells OP9