論文 - 詳細
RRC ID | 1269 |
---|---|
著者 | Kawamura T, Koka R, Ma A, Kumar V. |
タイトル | Differential roles for IL-15R alpha-chain in NK cell development and Ly-49 induction. |
ジャーナル | J Immunol |
Abstract |
IL-15Ralpha-deficient (IL-15Ralpha(-/-)) mice lack NK cells. However, when bone marrow (BM) progenitors from IL-15Ralpha(-/-) mice were cultured with IL-7, stem cell factor and flt3 ligand, followed by IL-15, they were able to differentiate into functional NK cells, indicating that IL-15Ralpha is not critical for NK cell development. Whereas NK cells generated in vitro from IL-15Ralpha(-/-) BM progenitors expressed CD94/NKG2, they failed to express Ly-49 receptors. In keeping with this, when IL-15Ralpha(-/-) BM cells were transferred into wild type recipients, they gave rise to NK cells in vivo, but with greatly reduced expression of Ly-49 receptors. Furthermore, the small numbers of NK cells found in IL-15(-/-) as well as IL-15Ralpha(-/-) but not flt3 ligand(-/-) mice expressed much lower levels of Ly-49 receptors than those from wild type mice. These results indicate a novel role for IL-15Ralpha-chain in Ly-49 induction on developing NK cells. |
巻・号 | 171(10) |
ページ | 5085-90 |
公開日 | 2003-11-15 |
DOI | 10.4049/jimmunol.171.10.5085 |
PMID | 14607906 |
MeSH | Animals Antigens, Ly / biosynthesis* Antigens, Ly / metabolism Bone Marrow Cells / cytology Bone Marrow Cells / immunology Bone Marrow Cells / metabolism Bone Marrow Transplantation / immunology Cell Differentiation / genetics Cell Differentiation / immunology Cells, Cultured Down-Regulation / genetics Down-Regulation / immunology Hematopoietic Stem Cells / cytology Hematopoietic Stem Cells / immunology Hematopoietic Stem Cells / metabolism Interleukin-15 / deficiency Interleukin-15 / genetics Interleukin-15 / metabolism* Killer Cells, Natural / cytology* Killer Cells, Natural / immunology* Killer Cells, Natural / metabolism Lectins, C-Type Mice Mice, Inbred C57BL Mice, Knockout Protein Subunits / biosynthesis Protein Subunits / deficiency Protein Subunits / genetics Protein Subunits / physiology* Radiation Chimera / genetics Radiation Chimera / immunology Receptors, Immunologic / antagonists & inhibitors Receptors, Immunologic / biosynthesis Receptors, Interleukin-15 Receptors, Interleukin-2 / biosynthesis Receptors, Interleukin-2 / deficiency Receptors, Interleukin-2 / genetics Receptors, Interleukin-2 / physiology* Receptors, NK Cell Lectin-Like |
IF | 4.886 |
引用数 | 43 |
WOS 分野 | IMMUNOLOGY |
リソース情報 | |
ヒト・動物細胞 | OP9(RCB1124) |