| RRC ID |
1273
|
| 著者 |
Li TC, Takeda N, Kato K, Nilsson J, Xing L, Haag L, Cheng RH, Miyamura T.
|
| タイトル |
Characterization of self-assembled virus-like particles of human polyomavirus BK generated by recombinant baculoviruses.
|
| ジャーナル |
Virology
|
| Abstract |
The major structural protein of the human polyomavirus BK (BKV), VP1, was expressed by using recombinant baculoviruses. A large amount of protein with a molecular mass of about 42 kDa was synthesized and identified by Western blotting. The protein was detected exclusively in the nuclei by immunofluorescent analysis and it was released into culture medium. The expressed BKV VP1 protein was self-assembled into virus-like particles (BK-VLPs) with two different sizes (50 and 26 nm in diameter), which migrated into four different bands in CsCl gradient with buoyant densities of 1.29, 1.30, 1.33, and 1.35 g/cm(3). The immunological studies on the BK-VLPs suggested that they have similar antigenicity with those of authentic BKV particles. Cryoelectron microscopy and 3D image analysis further revealed that the larger BK-VLPs were composed of 72 capsomers which all were pentamers arranged in a T = 7 surface lattice. This system provides useful information for detailed studies of viral morphogenesis and the structural basis for the antigenicity of BKV.
|
| 巻・号 |
311(1)
|
| ページ |
115-24
|
| 公開日 |
2003-6-20
|
| DOI |
10.1016/s0042-6822(03)00141-7
|
| PII |
S0042682203001417
|
| PMID |
12832209
|
| MeSH |
Amino Acid Sequence
Animals
BK Virus / chemistry
BK Virus / physiology*
BK Virus / ultrastructure
Baculoviridae / genetics*
Capsid / chemistry
Capsid Proteins / biosynthesis
Capsid Proteins / chemistry
Capsid Proteins / genetics*
Cell Line
Cell Nucleus / metabolism
Cloning, Molecular
Cryoelectron Microscopy
Culture Media
Genetic Vectors*
Humans
Image Processing, Computer-Assisted
Insecta
Models, Molecular
Molecular Sequence Data
Molecular Weight
Recombinant Proteins / biosynthesis
Virus Assembly
|
| IF |
2.819
|
| 引用数 |
41
|
|
WOS 分野
|
VIROLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Sf9(RCB0563) |
