RRC ID 12762
Author Fujita K, Yamaguchi Y, Mori T, Muramatsu N, Miyamoto T, Yano M, Miyata H, Ootsuyama A, Sawada M, Matsuda H, Kaji R, Sakaguchi S.
Title Effects of a brain-engraftable microglial cell line expressing anti-prion scFv antibodies on survival times of mice infected with scrapie prions.
Journal Cell Mol Neurobiol
Abstract We first verified that a single chain Fv fragment against prion protein (anti-PrP scFv) was secreted by HEK293T cells and prevented prion replication in infected cells. We then stably expressed anti-PrP scFv in brain-engraftable murine microglial cells and intracerebrally injected these cells into mice before or after infection with prions. Interestingly, the injection before or at an early time point after infection attenuated the infection marginally but significantly prolonged survival times of the mice. These suggest that the ex vivo gene transfer of anti-PrP scFvs using brain-engraftable cells could be a possible immunotherapeutic approach against prion diseases.
Volume 31(7)
Pages 999-1008
Published 2011-10-1
DOI 10.1007/s10571-011-9696-z
PMID 21516351
MeSH Animals Brain / cytology* Cell Line Genetic Vectors HEK293 Cells Humans Mice Microglia / cytology Microglia / physiology* Microglia / transplantation* Prions / immunology* Prions / pathogenicity Prions / physiology Recombinant Proteins / genetics Recombinant Proteins / immunology Scrapie / physiopathology* Scrapie / therapy Single-Chain Antibodies / genetics Single-Chain Antibodies / immunology* Single-Chain Antibodies / therapeutic use Survival Rate
IF 3.606
Times Cited 12
DNA material CS-CA-GFP (RDB05964) CS-CA-MCS (RDB05963) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393)