RRC ID 1297
著者 Wada W, Maeshima A, Zhang YQ, Hasegawa Y, Kuwano H, Kojima I.
タイトル Assessment of the function of the betaC-subunit of activin in cultured hepatocytes.
ジャーナル Am J Physiol Endocrinol Metab
Abstract We assessed the function of the beta(C)-subunit of activin in hepatocytes. We studied the effect of conditioned medium of Chinese hamster ovary (CHO) cell line stably expressing the beta(C) gene (CHO-beta(C)) on growth of AML12 hepatocytes. We also examined the effect of recombinant activin C and transfection of the beta(C) gene by using adenovirus vector. CHO-beta(C) secreted activin C, a homodimer of the beta(C), as well as precursors of the beta(C). The conditioned medium of CHO-beta(C) increased both [(3)H]thymidine incorporation and the cell number in AML12 cells. It also supported survival of AML12 cells in a serum-free condition. Recombinant human activin C also increased both [(3)H]thymidine incorporation and the number of AML12 cells. Transfection of AML12 cells with the beta(C)-subunit led to the stimulation of [(3)H]thymidine incorporation. Analysis of the conditioned medium revealed that the beta(C)-subunit formed a heterodimer with the endogenous beta(A), the formation of which was dependent on the amount of beta(C) expressed. Recombinant activin C did not affect the binding of (125)I-activin A to its receptor or follistatin. These results indicate that activin C stimulates growth of AML12 cells. The beta(C)-subunit modifies the function of the beta(A)-subunit by multiple mechanisms.
巻・号 287(2)
ページ E247-54
公開日 2004-8-1
DOI 10.1152/ajpendo.00390.2003
PII 00390.2003
PMID 15039147
MeSH Animals CHO Cells / metabolism Cell Division / physiology Cell Survival / physiology Cells, Cultured Cricetinae Cricetulus Culture Media, Conditioned / metabolism Hepatocytes / cytology Hepatocytes / metabolism* Humans Inhibin-beta Subunits / biosynthesis Inhibin-beta Subunits / physiology* Mice Protein Conformation Recombinant Proteins Transfection
IF 3.469
引用数 21
WOS 分野 PHYSIOLOGY ENDOCRINOLOGY & METABOLISM
リソース情報
ヒト・動物細胞 CHO-K1(RCB0285)