論文 - 詳細
| RRC ID | 1298 |
|---|---|
| 著者 | Wen XZ, Miyake S, Akiyama Y, Yuasa Y. |
| タイトル | BMP-2 modulates the proliferation and differentiation of normal and cancerous gastric cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Bone morphogenetic protein 2 (BMP-2), a member of the transforming growth factor beta super-family, has been shown to act as an antiproliferative agent for a variety of cell lines by activating signaling cascades that cause cell cycle arrest. However, the biological effect and mechanism of action of BMP-2 on gastric cells remain unknown. In the present study, we showed that recombinant human BMP-2 dose-dependently inhibited the growth of OUMS37 rat gastric cells and MKN74 human gastric cancer cells. The antiproliferation seems to be due to cell cycle arrest in the G1-phase, which was revealed by flow cytometric assays. BMP-2 increased the level of p21/WAF1/CIP1, suggesting that BMP-2-mediated inhibition of cell proliferation may be induced through p21/WAF1/CIP1. In addition, BMP-2 increased the expression of pepsinogen II, a differentiation marker of the stomach, in MKN74 cells. These results indicate that BMP-2 plays important roles in modulating the proliferation and differentiation of gastric epithelial cells. |
| 巻・号 | 316(1) |
| ページ | 100-6 |
| 公開日 | 2004-3-26 |
| DOI | 10.1016/j.bbrc.2004.02.016 |
| PII | S0006291X04002402 |
| PMID | 15003517 |
| MeSH | Animals Antineoplastic Agents / pharmacology* Bone Morphogenetic Protein 2 Bone Morphogenetic Protein Receptors Bone Morphogenetic Proteins / pharmacology* Cell Differentiation / drug effects Cell Division / drug effects Cell Line Cell Line, Tumor Cyclin-Dependent Kinase Inhibitor p21 Cyclins / biosynthesis Cyclins / genetics G1 Phase / drug effects Gastric Mucosa / cytology Gastric Mucosa / drug effects* Gastric Mucosa / metabolism Growth Inhibitors / pharmacology* Humans Pepsinogen A / biosynthesis Pepsinogen A / genetics Rats Receptors, Growth Factor / metabolism Recombinant Proteins Stomach Neoplasms / genetics Stomach Neoplasms / metabolism* Stomach Neoplasms / pathology Transforming Growth Factor beta* Up-Regulation |
| IF | 2.985 |
| 引用数 | 71 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | MKN74(RCB1002) |