RRC ID 1354
著者 Nishimaki H, Kasai K, Kozaki Ki, Takeo T, Ikeda H, Saga S, Nitta M, Itoh G.
タイトル A role of activated Sonic hedgehog signaling for the cellular proliferation of oral squamous cell carcinoma cell line.
ジャーナル Biochem Biophys Res Commun
Abstract Sonic hedgehog (Shh) is a secreted morphogen crucial for appropriate cellular proliferation during mammalian development. The activated Shh signaling is known to predispose to human tumors such as medulloblastoma and basal cell carcinoma, while a role of Shh signaling in the other common tumors is still controversial. Here we showed the overexpression of Shh in five cell lines among 14 human oral squamous cell carcinoma (OSCC) cell lines. One of the Shh-expressing OSCC cell lines HSQ-89 showed the inhibition of G1/S transition and apoptotic cell death by treatment with Cyclopamine, a steroidal alkaloid that blocks the intracellular Shh signaling. Furthermore, we found that treatment with Y-27632, a specific inhibitor of Rho-associated kinase, mimicked the effect of Cyclopamine on the cell cycle progression of HSQ-89. Our study revealed the involvement of activated Shh signaling in the cellular proliferation of OSCC cells, indicating Shh signaling might be a good therapeutic target for OSCC.
巻・号 314(2)
ページ 313-20
公開日 2004-2-6
DOI 10.1016/j.bbrc.2003.12.097
PII S0006291X03027281
PMID 14733907
MeSH Amides / pharmacology Apoptosis Blotting, Western Carcinoma, Squamous Cell / metabolism Cell Cycle Cell Division Cell Line, Tumor Cell Separation Culture Media, Conditioned / pharmacology Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology Flow Cytometry G1 Phase Hedgehog Proteins Humans Intracellular Signaling Peptides and Proteins Mouth Neoplasms / metabolism Protein Serine-Threonine Kinases / metabolism Pyridines / pharmacology S Phase Signal Transduction Time Factors Trans-Activators / metabolism* Veratrum Alkaloids / pharmacology rho-Associated Kinases
IF 2.985
引用数 52
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 HSQ-89(RCB0789) Sa3(RCB0980) SCCKN(RCB0441) SCCTF(RCB0442) T3M-1 clone2(RCB1015) T3M-1 cl-10(RCB1017)