論文 - 詳細
| RRC ID | 1415 |
|---|---|
| 著者 | Inanami O, Sugihara K, Okui T, Hayashi M, Tsujitani M, Kuwabara M. |
| タイトル | Hypoxia and etanidazole alter radiation-induced apoptosis in HL60 cells but not in MOLT-4 cells. |
| ジャーナル | Int J Radiat Biol |
| Abstract |
PURPOSE:To examine how hypoxia influences ionizing irradiation-induced apoptosis in cultured mammalian cells and how a hypoxic cell radiosensitizer sensitizes apoptosis under hypoxic conditions. MATERIALS AND METHODS:Two cell lines derived from human lymphocytes, HL60 and MOLT-4, were exposed to 15 Gy X-rays under aerobic and hypoxic conditions. Etanidazole was used as a hypoxic cell radiosensitizer. The apoptotic morphological changes of nuclei and the induction of ladder-like DNA fragmentation were assessed by fluorescence microscopy and agarose gel electrophoresis, respectively. RESULTS:In HL60 cells, apototic cell death and the activation of caspases 8, 9 and 3 were less induced under the hypoxic conditions than under the aerobic ones. Treatment of hypoxic cells with etanidazole enhanced X-ray-induced apoptosis and caspase activation. However, in MOLT-4 cells, neither hypoxia nor etanidazole influenced X-ray-induced apoptosis and caspase activation. In both cell lines, the frequency of X-ray-induced DNA double-strand breaks (DSB) under hypoxia was significantly smaller than that in aerobic conditions. Treatment of hypoxic cells with etanidazole enhanced them. CONCLUSION:These results suggested that X-ray-induced apoptosis in HL60 cells was initiated by DNA DSB and the treatment of hypoxic cells with etanidazole sensitized them through the enhancement of DSB induction, whereas X-ray-induced apoptosis in MOLT-4 cells occurred through damage other than to DNA. |
| 巻・号 | 78(4) |
| ページ | 267-74 |
| 公開日 | 2002-4-1 |
| DOI | 10.1080/09553000110105695 |
| PMID | 12020438 |
| MeSH | Apoptosis / drug effects* Apoptosis / radiation effects* Bromodeoxyuridine / pharmacology Caspases / metabolism Cell Hypoxia / physiology* Cell Line DNA Damage Enzyme Activation / drug effects Enzyme Activation / radiation effects Etanidazole / pharmacology* HL-60 Cells Humans Radiation Tolerance / drug effects Radiation-Sensitizing Agents / pharmacology* |
| IF | 2.368 |
| リソース情報 | |
| ヒト・動物細胞 | MOLT-4(RCB0206) |