論文 - 詳細
| RRC ID | 1440 |
|---|---|
| 著者 | Mustonen H, Lepistö A, Lehtonen S, Lehtonen E, Puolakkainen P, Kivilaakso E. |
| タイトル | CD2AP contributes to cell migration and adhesion in cultured gastric epithelium. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
The potential association of CD2AP with the adherens junction protein E-cadherin, co-localization with the actin cytoskeleton, and involvement in cell migration was investigated in cultured rat gastric mucosal cells. In stationary cells, CD2AP was localized perinuclearly while E-cadherin was expressed along cell-cell contacts and F-actin formed a branched network and adhesion belts. In migrating cells, CD2AP appeared as thread-like accumulations in the leading edges, colocalizing with F-actin and occasionally with E-cadherin. Intracellular injection of anti-CD2AP significantly retarded the migration speed of the cells suggesting a crucial role for CD2AP in mucosal cell migration, possibly as a scaffolding protein between cell membrane proteins and actin cytoskeleton. Co-immunoprecipitation assays revealed that CD2AP and E-cadherin are in a complex in HGF stimulated cells. It is concluded that CD2AP interacts with E-cadherin and co-localizes with F-actin in the leading edge of migrating cells, and significantly contributes to cell migration in restituting gastric epithelium. |
| 巻・号 | 332(2) |
| ページ | 426-32 |
| 公開日 | 2005-7-1 |
| DOI | 10.1016/j.bbrc.2005.04.140 |
| PII | S0006-291X(05)00922-8 |
| PMID | 15910750 |
| MeSH | Adaptor Proteins, Vesicular Transport / metabolism* Animals Cadherins / metabolism* Cell Movement / physiology Cells, Cultured Epithelial Cells / physiology* Gastric Mucosa / physiology* Rats |
| IF | 2.985 |
| 引用数 | 9 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | RGM1(RCB0876) |