RRC ID 1440
Author Mustonen H, Lepistö A, Lehtonen S, Lehtonen E, Puolakkainen P, Kivilaakso E.
Title CD2AP contributes to cell migration and adhesion in cultured gastric epithelium.
Journal Biochem Biophys Res Commun
Abstract The potential association of CD2AP with the adherens junction protein E-cadherin, co-localization with the actin cytoskeleton, and involvement in cell migration was investigated in cultured rat gastric mucosal cells. In stationary cells, CD2AP was localized perinuclearly while E-cadherin was expressed along cell-cell contacts and F-actin formed a branched network and adhesion belts. In migrating cells, CD2AP appeared as thread-like accumulations in the leading edges, colocalizing with F-actin and occasionally with E-cadherin. Intracellular injection of anti-CD2AP significantly retarded the migration speed of the cells suggesting a crucial role for CD2AP in mucosal cell migration, possibly as a scaffolding protein between cell membrane proteins and actin cytoskeleton. Co-immunoprecipitation assays revealed that CD2AP and E-cadherin are in a complex in HGF stimulated cells. It is concluded that CD2AP interacts with E-cadherin and co-localizes with F-actin in the leading edge of migrating cells, and significantly contributes to cell migration in restituting gastric epithelium.
Volume 332(2)
Pages 426-32
Published 2005-7-1
DOI 10.1016/j.bbrc.2005.04.140
PII S0006-291X(05)00922-8
PMID 15910750
MeSH Adaptor Proteins, Vesicular Transport / metabolism* Animals Cadherins / metabolism* Cell Movement / physiology Cells, Cultured Epithelial Cells / physiology* Gastric Mucosa / physiology* Rats
IF 2.985
Times Cited 9
Human and Animal Cells RGM1(RCB0876)