| RRC ID |
1440
|
| Author |
Mustonen H, Lepistö A, Lehtonen S, Lehtonen E, Puolakkainen P, Kivilaakso E.
|
| Title |
CD2AP contributes to cell migration and adhesion in cultured gastric epithelium.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
The potential association of CD2AP with the adherens junction protein E-cadherin, co-localization with the actin cytoskeleton, and involvement in cell migration was investigated in cultured rat gastric mucosal cells. In stationary cells, CD2AP was localized perinuclearly while E-cadherin was expressed along cell-cell contacts and F-actin formed a branched network and adhesion belts. In migrating cells, CD2AP appeared as thread-like accumulations in the leading edges, colocalizing with F-actin and occasionally with E-cadherin. Intracellular injection of anti-CD2AP significantly retarded the migration speed of the cells suggesting a crucial role for CD2AP in mucosal cell migration, possibly as a scaffolding protein between cell membrane proteins and actin cytoskeleton. Co-immunoprecipitation assays revealed that CD2AP and E-cadherin are in a complex in HGF stimulated cells. It is concluded that CD2AP interacts with E-cadherin and co-localizes with F-actin in the leading edge of migrating cells, and significantly contributes to cell migration in restituting gastric epithelium.
|
| Volume |
332(2)
|
| Pages |
426-32
|
| Published |
2005-7-1
|
| DOI |
10.1016/j.bbrc.2005.04.140
|
| PII |
S0006-291X(05)00922-8
|
| PMID |
15910750
|
| MeSH |
Adaptor Proteins, Vesicular Transport / metabolism*
Animals
Cadherins / metabolism*
Cell Movement / physiology
Cells, Cultured
Epithelial Cells / physiology*
Gastric Mucosa / physiology*
Rats
|
| IF |
2.985
|
| Times Cited |
9
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
RGM1(RCB0876) |
