RRC ID |
1440
|
著者 |
Mustonen H, Lepistö A, Lehtonen S, Lehtonen E, Puolakkainen P, Kivilaakso E.
|
タイトル |
CD2AP contributes to cell migration and adhesion in cultured gastric epithelium.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
The potential association of CD2AP with the adherens junction protein E-cadherin, co-localization with the actin cytoskeleton, and involvement in cell migration was investigated in cultured rat gastric mucosal cells. In stationary cells, CD2AP was localized perinuclearly while E-cadherin was expressed along cell-cell contacts and F-actin formed a branched network and adhesion belts. In migrating cells, CD2AP appeared as thread-like accumulations in the leading edges, colocalizing with F-actin and occasionally with E-cadherin. Intracellular injection of anti-CD2AP significantly retarded the migration speed of the cells suggesting a crucial role for CD2AP in mucosal cell migration, possibly as a scaffolding protein between cell membrane proteins and actin cytoskeleton. Co-immunoprecipitation assays revealed that CD2AP and E-cadherin are in a complex in HGF stimulated cells. It is concluded that CD2AP interacts with E-cadherin and co-localizes with F-actin in the leading edge of migrating cells, and significantly contributes to cell migration in restituting gastric epithelium.
|
巻・号 |
332(2)
|
ページ |
426-32
|
公開日 |
2005-7-1
|
DOI |
10.1016/j.bbrc.2005.04.140
|
PII |
S0006-291X(05)00922-8
|
PMID |
15910750
|
MeSH |
Adaptor Proteins, Vesicular Transport / metabolism*
Animals
Cadherins / metabolism*
Cell Movement / physiology
Cells, Cultured
Epithelial Cells / physiology*
Gastric Mucosa / physiology*
Rats
|
IF |
2.985
|
引用数 |
9
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
RGM1(RCB0876) |