RRC ID 1440
著者 Mustonen H, Lepistö A, Lehtonen S, Lehtonen E, Puolakkainen P, Kivilaakso E.
タイトル CD2AP contributes to cell migration and adhesion in cultured gastric epithelium.
ジャーナル Biochem Biophys Res Commun
Abstract The potential association of CD2AP with the adherens junction protein E-cadherin, co-localization with the actin cytoskeleton, and involvement in cell migration was investigated in cultured rat gastric mucosal cells. In stationary cells, CD2AP was localized perinuclearly while E-cadherin was expressed along cell-cell contacts and F-actin formed a branched network and adhesion belts. In migrating cells, CD2AP appeared as thread-like accumulations in the leading edges, colocalizing with F-actin and occasionally with E-cadherin. Intracellular injection of anti-CD2AP significantly retarded the migration speed of the cells suggesting a crucial role for CD2AP in mucosal cell migration, possibly as a scaffolding protein between cell membrane proteins and actin cytoskeleton. Co-immunoprecipitation assays revealed that CD2AP and E-cadherin are in a complex in HGF stimulated cells. It is concluded that CD2AP interacts with E-cadherin and co-localizes with F-actin in the leading edge of migrating cells, and significantly contributes to cell migration in restituting gastric epithelium.
巻・号 332(2)
ページ 426-32
公開日 2005-7-1
DOI 10.1016/j.bbrc.2005.04.140
PII S0006-291X(05)00922-8
PMID 15910750
MeSH Adaptor Proteins, Vesicular Transport / metabolism* Animals Cadherins / metabolism* Cell Movement / physiology Cells, Cultured Epithelial Cells / physiology* Gastric Mucosa / physiology* Rats
IF 2.985
引用数 9
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 RGM1(RCB0876)