RRC ID 1479
Author Ikeno Y, Konno N, Cheon SH, Bolchi A, Ottonello S, Kitamoto K, Arioka M.
Title Secretory phospholipases A2 induce neurite outgrowth in PC12 cells through lysophosphatidylcholine generation and activation of G2A receptor.
Journal J Biol Chem
Abstract We previously demonstrated that secretory phospholipase A2 (sPLA2) and lysophosphatidylcholine (LPC) exhibit neurotrophin-like neuritogenic activity in the rat pheochromocytoma cell line PC12. In this study, we further analyzed the mechanism whereby sPLA2 displays neurite-inducing activity. Exogenously added mammalian group X sPLA2 (sPLA2-X), but not group IB and IIA sPLA2s, induced neuritogenesis, which correlated with the ability of sPLA2-X to liberate LPC into the culture media. In accordance, blocking the effect of LPC by supplementation of bovine serum albumin or phospholipase B attenuated neuritogenesis by sPLA2 or LPC. Overproduction or suppression of G2A, a G-protein-coupled receptor involved in LPC signaling, resulted in the enhancement or reduction of neuritogenesis induced by sPLA2 treatment. These results indicate that the neuritogenic effect of sPLA2 is mediated by generation of LPC and subsequent activation of G2A.
Volume 280(30)
Pages 28044-52
Published 2005-7-29
DOI 10.1074/jbc.M503343200
PII S0021-9258(20)56659-3
PMID 15927955
MeSH Adenoviridae / genetics Animals Calcium Channels, L-Type / metabolism Cattle Cell Cycle Proteins / metabolism* Cloning, Molecular Culture Media / metabolism DNA, Complementary / metabolism Dose-Response Relationship, Drug Flow Cytometry Genetic Vectors Green Fluorescent Proteins / metabolism Group II Phospholipases A2 Group X Phospholipases A2 Immunoblotting Lysophosphatidylcholines / metabolism* Lysophospholipase / metabolism Mice Mutation Neurons / metabolism* Nicardipine / pharmacology PC12 Cells Phospholipases A / metabolism Phospholipases A / physiology* Phospholipases A2 Polymerase Chain Reaction Potassium / metabolism Protein Binding RNA Interference Rats Receptors, G-Protein-Coupled / metabolism* Reverse Transcriptase Polymerase Chain Reaction Serum Albumin / metabolism Signal Transduction Time Factors
IF 4.238
Times Cited 45
DNA material pAxCAwtit (RDB03121)
Human and Animal Cells 293(RCB1637)