| RRC ID |
148
|
| 著者 |
Satoh M, Haruta-Satoh E, Omori A, Oh-Ishi M, Kodera Y, Furudate S, Maeda T.
|
| タイトル |
Effect of thyroxine on abnormal pancreatic proteomes of the hypothyroid rdw rat.
|
| ジャーナル |
Proteomics
|
| Abstract |
A mutation in the thyroglobulin (Tg) gene is the primary cause of hereditary dwarfism and hypothyroidism in the rdw rat. Despite the Tg mutation that causes a Tg shortage, rdw rats survive. The present study examines the influences of this condition on the pancreatic proteome. Normal control (group 1; n = 19) and rdw rats that did not receive L-thyroxine (T4) (group 2; n = 27) were sacrificed from 4 to 56 weeks after birth. The rdw rats were supplemented either with daily intraperitoneal injections of T4 from 3 to 28 days after birth (group 3; n = 4) or with normal thyroid tissues grafted at 4 weeks of age (group 4; n = 3). Groups 3 and 4 were sacrificed 12 weeks after birth. Pancreatic proteomes analyzed by two-dimensional gel electrophoresis showed that levels of at least four pancreatic proteins were higher in group 2 than in group 1, and that those of four were lower. Cluster decomposition and principal component analysis of the eight protein contents showed that groups 1 and 2 were separated into two clusters and that pancreatic proteomes of group 4 were better normalized than those of group 3. Injecting T4 into group 3 was temporarily effective, whereas the thyroid graft to group 4 provided a continuous positive effect, which concurred with the increased body weight of the other two groups of rdw rats that received grafts of normal thyroids.
|
| 巻・号 |
5(4)
|
| ページ |
1113-24
|
| 公開日 |
2005-3-1
|
| DOI |
10.1002/pmic.200401117
|
| PMID |
15712237
|
| MeSH |
Animals
Body Weight
Cluster Analysis
Disease Models, Animal
Electrophoresis, Gel, Two-Dimensional
Female
Genetic Markers
Growth Hormone / metabolism
Heterozygote
Hypothyroidism / metabolism*
Image Processing, Computer-Assisted
Male
Mass Spectrometry
Multivariate Analysis
Mutation
Pancreas / drug effects*
Pancreas / metabolism*
Proteomics / methods*
Rats
Rats, Mutant Strains
Receptors, Thyroid Hormone / metabolism
Sequence Analysis, Protein
Thyroid Gland / metabolism
Thyroxine / pharmacology*
Time Factors
|
| IF |
3.254
|
| 引用数 |
18
|
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ラット |
WIC-Tgrdw/Kts(strainID=193) |
