RRC ID 1492
Author Thinyane K, Baier PC, Schindehütte J, Mansouri A, Paulus W, Trenkwalder C, Flügge G, Fuchs E.
Title Fate of pre-differentiated mouse embryonic stem cells transplanted in unilaterally 6-hydroxydopamine lesioned rats: histological characterization of the grafted cells.
Journal Brain Res
Abstract We transplanted mouse embryonic stem (ES) cells pre-differentiated on a PA6 feeder cell layer into the striatum of 6-hydroxydopamine hemi-lesioned adult rats and studied the fate of the grafted cells 1 and 5 weeks post-grafting. At both time points, ES cell grafts contained tyrosine hydroxylase positive (TH+) and 5-HT immunoreactive cells. Between 1 and 5 weeks, there was an enlargement of the grafts and an increase in number of TH+ cells although the differences between the two time points were not significant. The mean number of TH+ neurons per striatum was 330 +/- 73 after 1 week and 1220 +/- 400 after 5 weeks. Over the same time period, mean soma profile area of the TH+ neurons increased significantly by 25.2%. Neurites were longer after 5 weeks (by 24.9%), but the difference to 1 week post-grafting was not reliable. The percentage of TH+ somata without neurites increased from 6.7% after 1 week to 38.3% after 5 weeks (not significant). After 5 weeks, two out of fifteen graft recipients had tumors indicating that pre-differentiation of mouse embryonic stem cells using this differentiation protocol is not sufficient to prevent tumor formation.
Volume 1045(1-2)
Pages 80-7
Published 2005-5-31
DOI 10.1016/j.brainres.2005.03.033
PII S0006-8993(05)00455-5
PMID 15910765
MeSH Animals Biomarkers / metabolism Brain Neoplasms / etiology Brain Neoplasms / pathology Brain Neoplasms / physiopathology Cell Count Cell Differentiation / physiology* Cell Lineage / physiology Cell Proliferation Cell Transformation, Neoplastic / metabolism Corpus Striatum / cytology Corpus Striatum / physiology Corpus Striatum / surgery Disease Models, Animal Graft Survival / physiology* Male Mice Neurites / physiology Neurites / ultrastructure Neurons / cytology Neurons / metabolism Oxidopamine Parkinsonian Disorders / therapy* Pluripotent Stem Cells / cytology Pluripotent Stem Cells / metabolism* Rats Rats, Wistar Stem Cell Transplantation / adverse effects Stem Cell Transplantation / methods* Transplantation, Heterologous Tyrosine 3-Monooxygenase / metabolism
IF 2.733
Times Cited 22
Human and Animal Cells MC3T3-G2/PA6(RCB1127)