RRC ID |
1492
|
著者 |
Thinyane K, Baier PC, Schindehütte J, Mansouri A, Paulus W, Trenkwalder C, Flügge G, Fuchs E.
|
タイトル |
Fate of pre-differentiated mouse embryonic stem cells transplanted in unilaterally 6-hydroxydopamine lesioned rats: histological characterization of the grafted cells.
|
ジャーナル |
Brain Res
|
Abstract |
We transplanted mouse embryonic stem (ES) cells pre-differentiated on a PA6 feeder cell layer into the striatum of 6-hydroxydopamine hemi-lesioned adult rats and studied the fate of the grafted cells 1 and 5 weeks post-grafting. At both time points, ES cell grafts contained tyrosine hydroxylase positive (TH+) and 5-HT immunoreactive cells. Between 1 and 5 weeks, there was an enlargement of the grafts and an increase in number of TH+ cells although the differences between the two time points were not significant. The mean number of TH+ neurons per striatum was 330 +/- 73 after 1 week and 1220 +/- 400 after 5 weeks. Over the same time period, mean soma profile area of the TH+ neurons increased significantly by 25.2%. Neurites were longer after 5 weeks (by 24.9%), but the difference to 1 week post-grafting was not reliable. The percentage of TH+ somata without neurites increased from 6.7% after 1 week to 38.3% after 5 weeks (not significant). After 5 weeks, two out of fifteen graft recipients had tumors indicating that pre-differentiation of mouse embryonic stem cells using this differentiation protocol is not sufficient to prevent tumor formation.
|
巻・号 |
1045(1-2)
|
ページ |
80-7
|
公開日 |
2005-5-31
|
DOI |
10.1016/j.brainres.2005.03.033
|
PII |
S0006-8993(05)00455-5
|
PMID |
15910765
|
MeSH |
Animals
Biomarkers / metabolism
Brain Neoplasms / etiology
Brain Neoplasms / pathology
Brain Neoplasms / physiopathology
Cell Count
Cell Differentiation / physiology*
Cell Lineage / physiology
Cell Proliferation
Cell Transformation, Neoplastic / metabolism
Corpus Striatum / cytology
Corpus Striatum / physiology
Corpus Striatum / surgery
Disease Models, Animal
Graft Survival / physiology*
Male
Mice
Neurites / physiology
Neurites / ultrastructure
Neurons / cytology
Neurons / metabolism
Oxidopamine
Parkinsonian Disorders / therapy*
Pluripotent Stem Cells / cytology
Pluripotent Stem Cells / metabolism*
Rats
Rats, Wistar
Stem Cell Transplantation / adverse effects
Stem Cell Transplantation / methods*
Transplantation, Heterologous
Tyrosine 3-Monooxygenase / metabolism
|
IF |
2.733
|
引用数 |
22
|
WOS 分野
|
NEUROSCIENCES
|
リソース情報 |
ヒト・動物細胞 |
MC3T3-G2/PA6(RCB1127) |