論文 - 詳細
| RRC ID | 15294 |
|---|---|
| 著者 | Moriya H, Chino A, Kapuy O, Csikász-Nagy A, Novák B. |
| タイトル | Overexpression limits of fission yeast cell-cycle regulators in vivo and in silico. |
| ジャーナル | Mol Syst Biol |
| Abstract |
Cellular systems are generally robust against fluctuations of intracellular parameters such as gene expression level. However, little is known about expression limits of genes required to halt cellular systems. In this study, using the fission yeast Schizosaccharomyces pombe, we developed a genetic 'tug-of-war' (gTOW) method to assess the overexpression limit of certain genes. Using gTOW, we determined copy number limits for 31 cell-cycle regulators; the limits varied from 1 to >100. Comparison with orthologs of the budding yeast Saccharomyces cerevisiae suggested the presence of a conserved fragile core in the eukaryotic cell cycle. Robustness profiles of networks regulating cytokinesis in both yeasts (septation-initiation network (SIN) and mitotic exit network (MEN)) were quite different, probably reflecting differences in their physiologic functions. Fragility in the regulation of GTPase spg1 was due to dosage imbalance against GTPase-activating protein (GAP) byr4. Using the gTOW data, we modified a mathematical model and successfully reproduced the robustness of the S. pombe cell cycle with the model. |
| 巻・号 | 7 |
| ページ | 556 |
| 公開日 | 2011-12-6 |
| DOI | 10.1038/msb.2011.91 |
| PII | msb201191 |
| PMID | 22146300 |
| PMC | PMC3737731 |
| MeSH | Cell Cycle / genetics* Cell Cycle Proteins / genetics Computer Simulation Cytokinesis / genetics GTP Phosphohydrolases / genetics Gene Dosage Gene Expression Regulation, Fungal* Models, Genetic* Schizosaccharomyces / cytology Schizosaccharomyces / genetics* Schizosaccharomyces pombe Proteins / genetics Systems Biology |
| IF | 8.991 |
| 引用数 | 12 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
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オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY7652 |