Reference - Detail
| RRC ID | 15304 |
|---|---|
| Author | Fujita Y, Tohda H, Giga-Hama Y, Takegawa K. |
| Title | Heat shock-inducible expression vectors for use in Schizosaccharomyces pombe. |
| Journal | FEMS Yeast Res |
| Abstract |
A new, heat shock-inducible expression system based on an endogenous hsp16+ promoter was developed for use in the fission yeast Schizosaccharomyces pombe. Analysis of GFP expression profiles indicated that a 1.2-kb segment of the hsp16+ promoter region was sufficient to drive expression of heterologous protein. The hsp16+ promoter was found to be activated not only by heat shock but also by other stresses including cadmium, ethanol, and oxidative stress. Two expression vectors, pHIL and pHIU, were constructed using the 1.2-kb hsp16+ promoter for inducible gene expression in Sch. pombe. This new expression system utilizes a simple induction protocol and promises to be a useful tool for analyzing gene expression in Sch. pombe. |
| Volume | 6(6) |
| Pages | 883-7 |
| Published | 2006-9-1 |
| DOI | 10.1111/j.1567-1364.2006.00093.x |
| PII | FYR093 |
| PMID | 16911510 |
| MeSH | Cadmium / pharmacology Enzyme Activators / pharmacology Ethanol / pharmacology Gene Expression Gene Expression Regulation, Fungal Genes, Reporter Genetic Vectors* Green Fluorescent Proteins / biosynthesis Green Fluorescent Proteins / genetics Heat-Shock Proteins / genetics Hot Temperature Oxidative Stress Promoter Regions, Genetic Recombinant Proteins / biosynthesis* Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics |
| IF | 3.193 |
| Times Cited | 9 |
| WOS Category | MYCOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Yeast | FYP4216(pAL-SK) FYP4221(pAU-SK) |